Any feedback?
Literature summary for 3.4.11.22 extracted from
- A continuous fluorigenic assay for the measurement of the activity of endoplasmic reticulum aminopeptidase 1: competition kinetics as a tool for enzyme specificity investigation (2009), Anal. Biochem., 395, 33-40.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in insect cells driven by a baculoviral vector | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amastatin | about 50% inhibition at 0.1 mM | Homo sapiens |  |
| leucinethiol | about 80% inhibition at 0.1 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
WRVYEKCALK | at 37°C | Homo sapiens | |
| 0.328 | - |
WEVYEKCALK | at 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | ERAP1 trims a large variety of long peptide sequences efficiently, and its activity drops dramatically for peptides shorter than eight or nine amino acids, the enzyme efficiently excises virtually any amino acid in the context of a larger peptide, ERAP1 has broad N-terminal specificity due to strong preferences for residues downstream in the peptide-substrate sequence | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, MonoQ column chromatography, and Superdex S200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | ERAP1 trims a large variety of long peptide sequences efficiently, and its activity drops dramatically for peptides shorter than eight or nine amino acids, the enzyme efficiently excises virtually any amino acid in the context of a larger peptide, ERAP1 has broad N-terminal specificity due to strong preferences for residues downstream in the peptide-substrate sequence | Homo sapiens | ? | - |
? | |
| WEVYEKCALK + H2O | - |
Homo sapiens | L-Trp + EVYEKCALK | - |
? | |
| WRVYEKCALK + H2O | - |
Homo sapiens | L-Trp + RVYEKCALK | - |
? | |
| WRVYEKMALKC + H2O | - |
Homo sapiens | L-Trp + RVYEKMALKC | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoplasmic reticulum aminopeptidase 1 | - |
Homo sapiens |
| endoplasmic reticulum aminopeptidase associated with antigen presentation | - |
Homo sapiens |
| ERAAP | - |
Homo sapiens |
| ERAP1 | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
