Literature summary for 3.4.11.22 extracted from

Adachi, W.; Suzuki, N.N.; Fujioka, Y.; Suzuki, K.; Ohsumi, Y.; Inagaki, F.
Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway (2007), Acta Crystallogr. Sect. F, 63, 200-203.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method with 0.1 M Tris-HCl pH 6.5, 30% polyethylene glycol 400, 0.1 M MgCl2 and 1.1 M NaCl	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuole	-	Saccharomyces cerevisiae	5773	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
400000	-	beyond 400000 Da, gel filtration	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose 4B column chromatography, Superdex 200 gel filtration, Superdex 75 gel filtration, and Resource RPC column chromatography	Saccharomyces cerevisiae

Subunits

Subunits	Comment	Organism
dodecamer	x-ray crystallography	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
aminopeptidase 1	-	Saccharomyces cerevisiae
APE1	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)