Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tert-butanol | 20% activation at 10% at 20-90°C | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, no inhibition at pH 8.1, but conversion into the apoenzyme at pH 6.0 | Geobacillus stearothermophilus | |
Urea | at 8 M, inhibition of 7% of the enzyme activity with Gly-Leu-Tyr, but 75% of the activity with L-Leu-4-nitroanilide | Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme exists in a membrane and a soluble variant with slightly differing substrate specificities and thermostabilities | Geobacillus stearothermophilus | 16020 | - |
soluble | the enzyme exists in a membrane and a soluble variant with slightly differing substrate specificities and thermostabilities | Geobacillus stearothermophilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | binding results in much higher activity compared to Zn2+, Co2+ or Zn2+ is required for maximal activity, metallopeptidase | Geobacillus stearothermophilus | |
Zn2+ | binding results in much lower activity compared to Co2+, very tight binding, Co2+ or Zn2+ is required for maximal activity, metallopeptidase | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
12 * 36500, heterododecamer with two types of subunits with similar MW, the enzyme shows different hybrid types, overview, the enzymatic activity of the alpha-subunit differs from that of the beta-subunit | Geobacillus stearothermophilus |
400000 | - |
sedimentation equilibrium centrifugation analysis | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 19fold | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Leu-Tyr + H2O | - |
Geobacillus stearothermophilus | Gly + Leu-Tyr | - |
? | |
L-Leu-4-nitroanilide + H2O | substrate of the alpha-subunit, but not of the beta-subunit | Geobacillus stearothermophilus | L-Leu + 4-nitroaniline | - |
? | |
additional information | the alpha-subunits hydrolyzes peptides with neutral N-terminal amino acids while the beta-subunit acts on peptides with acidic N-terminal Glu or Asp residues | Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 36500, heterododecamer with two types of subunits with similar MW, the enzyme shows different hybrid types, overview, the enzymatic activity of the alpha-subunit differs from that of the beta-subunit | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
API | - |
Geobacillus stearothermophilus |
Bacillus aminopeptidase I | - |
Geobacillus stearothermophilus |
More | the enzyme belongs to the peptidase family M42 | Geobacillus stearothermophilus |
thermophilic aminopeptidase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
with substrate Gly-Leu-Tyr | Geobacillus stearothermophilus |
90 | - |
with substrate L-Leu-4-nitroanilide | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the apo- and cobalt-free enzyme is thermolabile | Geobacillus stearothermophilus |
80 | - |
loss of 20% activity within 30 min, stable for several hours | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | with substrate L-Leu-4-nitroanilide | Geobacillus stearothermophilus |
9.2 | 9.5 | with substrate Gly-Leu-Tyr | Geobacillus stearothermophilus |