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Literature summary for 3.4.11.22 extracted from
- Aminopeptidase I (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds), 1, 940-941.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene APE1, DNA and amino acid sequence determination and analysis, transcription is regulated by growth phase and by carbon source | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a mutant enzyme is defective in autophagocytosis and vacuole biogenesis | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | the zymogen remains in the cytoplasm and does not enter the secretory pathway | Saccharomyces cerevisiae | 5829 | - |
| vacuole | the precursor enzyme is transported into the vacuole via the non-classic Cvt pathway by cytoplasmic double-membrane vesicles, which fuse with the vacuole releasing a single-membrane autophagic body into the vacuolar lumen where it is proteolytically maturated, overview | Saccharomyces cerevisiae | 5773 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | specific, strong activation | Saccharomyces cerevisiae |  |
| Zn2+ | specific, strong activation, 2 mol per mol of enzyme | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
12 * 53000, active enzyme | Saccharomyces cerevisiae |
| 640000 | - |
- |
Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
gene APE1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the zymogen is unglycosylated | Saccharomyces cerevisiae |
| proteolytic modification | the inactive zymogen is activated by cleavage through cerevisin in a PEP4-dependent manner | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Leu-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | L-Leu + 4-nitroaniline | - |
? | |
| additional information | the enzyme prefers substrates with N-terminal leucine or another hydrophobic amino acid residue, and is less active with Gly or charged amino acids at the P1 position | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | 12 * 53000, active enzyme | Saccharomyces cerevisiae |
| More | dimeric and hexameric enzyme forms are inactive | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase I | - |
Saccharomyces cerevisiae |
| Aminopeptidase III | - |
Saccharomyces cerevisiae |
| aminopeptidase IV | - |
Saccharomyces cerevisiae |
| Aminopeptidase yscI | - |
Saccharomyces cerevisiae |
| LAP IV | - |
Saccharomyces cerevisiae |
| More | the enzyme belongs to the peptidase family M18 | Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
4.7 |
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