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Literature summary for 3.4.11.22 extracted from

  • Byun, T; Tang, M.; Sloma, A.; Brown, K.M.; Marumoto, C.; Fujii, M.; Blinkovsky, A.M.
    Aminopeptidase from Sphingomonas capsulata (2001), J. Biol. Chem., 276, 17902-17907.
    View publication on PubMed
Search for more literature for 3.4.11.22

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Bacillus subtilis and Escherichia coli Novosphingobium capsulatum

Inhibitors

Inhibitors Comment Organism Structure
ferrocyanide
-
 Novosphingobium capsulatum
iodate
-
 Novosphingobium capsulatum
additional information no inhibition by EDTA and PMSF Novosphingobium capsulatum
o-phenanthroline 96% inhibition Novosphingobium capsulatum
phosphate
-
 Novosphingobium capsulatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
 Ala beta-naphthylamide pH 7.5, 21°C, native enzyme Novosphingobium capsulatum
14
-
 Ala-4-nitroanilide pH 7.5, 21°C, native enzyme Novosphingobium capsulatum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion to culture medium Novosphingobium capsulatum
-
-
additional information not in the periplasm Novosphingobium capsulatum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no detectable content of calcium and cobalt Novosphingobium capsulatum
Zinc 1 atom per enzyme molecule, contains the HEXXH motif Novosphingobium capsulatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Novosphingobium capsulatum

Organism

Organism UniProt Comment Textmining
Novosphingobium capsulatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native from culture supernatant Novosphingobium capsulatum

Reaction

Reaction Comment Organism Reaction ID
Release of an N-terminal amino acid, preferably a neutral or hydrophobic one, from a polypeptide. Aminoacyl-arylamides are poor substrates Gly-Gly bond is preferably hydrolyzed Novosphingobium capsulatum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
 purified native enzyme, substrate Ala-4-nitroanilide Novosphingobium capsulatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-4-nitroanilide + H2O best Xaa-4-nitroanilide substrate Novosphingobium capsulatum Ala + 4-nitroaniline
-
 ?
Ala-4-nitroanilide + H2O best Xaa-4-nitroanilide substrate Novosphingobium capsulatum IFO 12533 Ala + 4-nitroaniline
-
 ?
Ala-Ala-Pro-Tyr-Lys-amide + H2O
-
 Novosphingobium capsulatum Ala + Ala-Pro-Tyr-Lys-amide
-
 ?
Ala-beta-naphthylamide + H2O
-
 Novosphingobium capsulatum Ala + beta-naphthylamine
-
 ?
Asp-4-nitroanilide + H2O
-
 Novosphingobium capsulatum Asp + 4-nitroaniline
-
 ?
Glu-4-nitroanilide + H2O
-
 Novosphingobium capsulatum Glu + 4-nitroaniline
-
 ?
Glu-Ala-Pro-Tyr-Lys-amide + H2O
-
 Novosphingobium capsulatum Glu + Ala-Pro-Tyr-Lys-amide
-
 ?
Gly-4-nitroanilide + H2O
-
 Novosphingobium capsulatum Gly + 4-nitroaniline
-
 ?
Gly-Ala-Pro-Tyr-Lys-amide + H2O best pentapeptide substrate Novosphingobium capsulatum Gly + Ala-Pro-Tyr-Lys-amide
-
 ?
Leu-4-nitroanilide + H2O
-
 Novosphingobium capsulatum Leu + 4-nitroaniline
-
 ?
Leu-4-nitroanilide + H2O
-
 Novosphingobium capsulatum IFO 12533 Leu + 4-nitroaniline
-
 ?
Leu-Ala-Pro-Tyr-Lys-amide + H2O
-
 Novosphingobium capsulatum Leu + Ala-Pro-Tyr-Lys-amide
-
 ?
leucine enkephalin + H2O recombinant enzyme, release of Tyr, Gly, Phe and Leu Novosphingobium capsulatum ?
-
 ?
Lys-4-nitroanilide + H2O low activity Novosphingobium capsulatum Lys + 4-nitroaniline
-
 ?
Lys-4-nitroanilide + H2O low activity Novosphingobium capsulatum IFO 12533 Lys + 4-nitroaniline
-
 ?
Met-4-nitroanilide + H2O
-
 Novosphingobium capsulatum Met + 4-nitroaniline
-
 ?
Met-4-nitroanilide + H2O
-
 Novosphingobium capsulatum IFO 12533 Met + 4-nitroaniline
-
 ?
additional information no activity with 4-nitroanilides of Pro, Ile, Val, and Phe Novosphingobium capsulatum ?
-
 ?
additional information no activity with 4-nitroanilides of Pro, Ile, Val, and Phe Novosphingobium capsulatum IFO 12533 ?
-
 ?
Pro-Ala-Pro-Tyr-Lys-amide + H2O
-
 Novosphingobium capsulatum Pro + Ala-Pro-Tyr-Lys-amide
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Novosphingobium capsulatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
 Novosphingobium capsulatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 20 min, loss of 40% activity Novosphingobium capsulatum
50
-
 20 min, complete loss of activity Novosphingobium capsulatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 Ala-beta-naphthylamide pH 7.5, 21°C, native enzyme Novosphingobium capsulatum
126.7
-
 Ala-4-nitroanilide pH 7.5, 21°C, native enzyme Novosphingobium capsulatum
138
-
 Ala-4-nitroanilide pH 7.5, 21°C, native enzyme Novosphingobium capsulatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4
-
 Novosphingobium capsulatum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3
-
 phosphate pH 7.5, 21°C Novosphingobium capsulatum
4.2
-
 ferrocyanide pH 7.5, 21°C Novosphingobium capsulatum
11
-
 iodate pH 7.5, 21°C Novosphingobium capsulatum