BRENDA - Reference to 3.4.11.22; Id = 28290

1,10 phenanthroline

-

Saccharomyces cerevisiae

amastatin

-

Saccharomyces cerevisiae

bestatin

-

Saccharomyces cerevisiae

EDTA

-

Saccharomyces cerevisiae

additional information

no inhibition by phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, N.ethylmaleimide, pepstatin, phosphoramidon, puromycin

Saccharomyces cerevisiae

0.0225

-

Cystinyl 4-nitroanilide

-

Saccharomyces cerevisiae

Co2+

CoCl2 activates

Saccharomyces cerevisiae

Zn2+

ZnCl2 has no influence on activity

Saccharomyces cerevisiae

45000

-

x * 45000, SDS-PAGE

Saccharomyces cerevisiae

52000

-

gel filtration, non-denaturing PAGE

Saccharomyces cerevisiae

-

no glycoprotein

no N-glycosylation

Saccharomyces cerevisiae

aminopeptidase yscXVI

Saccharomyces cerevisiae

4°C, 50 mM Tris-HCl buffer, pH 7.2, 38% loss of activity after 4 days, 81% loss of activity after 11 days

Saccharomyces cerevisiae

Ala 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Ala + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Arg 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Arg + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Cystinyl 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Cystine + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Glu 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Glu + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Gly 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Gly + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Leu 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Leu + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Lys 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Lys + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Met 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Met + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

additional information

no hydrolyis of succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, succinyl-Ala-Ala-Pro-Leu 4-nitroanilide, succinyl-Ala-Ala-Val- Ala 4-nitroanilide, benzyloxycarbonyl-Arg-Arg 4-nitroanilide, benzyloxycarbonyl-Gly-Pro 4-nitroanilide, benzyloxycarbonyl-Pro-Phe-Arg 4-nitroanilide, acetyl-Arg-Gly-Gly-Met 4-nitroanilide, benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide, benzoyl-Tyr 4-nitroanilide, benzyloxycarbonyl-Lys-Arg 4-nitroanilide

28290

Saccharomyces cerevisiae

?

-

Phe 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Phe + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Pro 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Pro + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

S-Benzoyl-Cys 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

S-Benzoyl-Cys + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Val 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Val + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

?

x * 45000, SDS-PAGE

Saccharomyces cerevisiae

7

8

substrate cystinyl 4-nitroanilide

Saccharomyces cerevisiae

4

9

no activity at pH 4 and pH 9, about 33% of maximal activity at pH 6

Saccharomyces cerevisiae

1,10 phenanthroline

-

Saccharomyces cerevisiae

amastatin

-

Saccharomyces cerevisiae

bestatin

-

Saccharomyces cerevisiae

EDTA

-

Saccharomyces cerevisiae

additional information

no inhibition by phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, N.ethylmaleimide, pepstatin, phosphoramidon, puromycin

Saccharomyces cerevisiae

0.0225

-

Cystinyl 4-nitroanilide

-

Saccharomyces cerevisiae

Co2+

CoCl2 activates

Saccharomyces cerevisiae

Zn2+

ZnCl2 has no influence on activity

Saccharomyces cerevisiae

45000

-

x * 45000, SDS-PAGE

Saccharomyces cerevisiae

52000

-

gel filtration, non-denaturing PAGE

Saccharomyces cerevisiae

no glycoprotein

no N-glycosylation

Saccharomyces cerevisiae

aminopeptidase yscXVI

Saccharomyces cerevisiae

4°C, 50 mM Tris-HCl buffer, pH 7.2, 38% loss of activity after 4 days, 81% loss of activity after 11 days

Saccharomyces cerevisiae

Ala 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Ala + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Arg 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Arg + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Cystinyl 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Cystine + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Glu 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Glu + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Gly 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Gly + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Leu 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Leu + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Lys 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Lys + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Met 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Met + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

additional information

no hydrolyis of succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, succinyl-Ala-Ala-Pro-Leu 4-nitroanilide, succinyl-Ala-Ala-Val- Ala 4-nitroanilide, benzyloxycarbonyl-Arg-Arg 4-nitroanilide, benzyloxycarbonyl-Gly-Pro 4-nitroanilide, benzyloxycarbonyl-Pro-Phe-Arg 4-nitroanilide, acetyl-Arg-Gly-Gly-Met 4-nitroanilide, benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide, benzoyl-Tyr 4-nitroanilide, benzyloxycarbonyl-Lys-Arg 4-nitroanilide

28290

Saccharomyces cerevisiae

?

-

Phe 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Phe + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Pro 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Pro + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

S-Benzoyl-Cys 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

S-Benzoyl-Cys + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

Val 4-nitroanilide + H2O

-

28290

Saccharomyces cerevisiae

Val + 4-nitroaniline

-

28290

Saccharomyces cerevisiae

-

?

x * 45000, SDS-PAGE

Saccharomyces cerevisiae

7

8

substrate cystinyl 4-nitroanilide

Saccharomyces cerevisiae

4

9

no activity at pH 4 and pH 9, about 33% of maximal activity at pH 6

Saccharomyces cerevisiae

732014

Goto

Substrate-dependent nitric oxi ...

Mus musculus

J. Biochem.

157

439-449

2015

-

1

-

1

-

1

-

2

-

3

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

732285

Aldhamen

Autoimmune disease-associated ...

Homo sapiens

J. Innate Immun.

7

275-289

2015

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

731234

Chen

Critical role of endoplasmic r ...

Homo sapiens

Arthritis Rheum.

66

284-294

2014

-

1

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

732737

Aldhamen

Endoplasmic reticulum aminopep ...

Mus musculus

PLoS ONE

8

e69539

2013

-

2

-

1

-

717825

Goto

Secretion of endoplasmic retic ...

Mus musculus

J. Biol. Chem.

286

21906-21914

2011

-

1

-

2

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

718148

Kim

Human cytomegalovirus microRNA ...

Homo sapiens

Nat. Immunol.

12

984-991

2011

-

1

-

2

-

1

-

3

-

1

-

1

-

3

-

1

-

718329

Kochan

Crystal structures of the endo ...

Homo sapiens

Proc. Natl. Acad. Sci. USA

108

7745-7750

2011

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

707156

Tsui

Association of an ERAP1 ERAP2 ...

Homo sapiens

Ann. Rheum. Dis.

69

733-736

2010

-

1

-

1

-

1

-

707111

Evnouchidou

A continuous fluorigenic assay ...

Homo sapiens

Anal. Biochem.

395

33-40

2009

-

1

-

2

-

1

-

1

-

1

-

4

-

1

-

2

-

2

-

1

-

1

-

4

-

707315

Maksymowych

Association of a specific ERAP ...

Homo sapiens

Arthritis Rheum.

60

1317-1323

2009

-

1

-

2

-

1

-

707657

Adamis

Lap4, a vacuolar aminopeptidas ...

Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741

Biometals

22

243-249

2009

-

1

-

55

-

1

-

2

-

708255

Evnouchidou

A new role for Zn(II) aminopep ...

Mus musculus

Curr. Pharm. Des.

15

3656-3670

2009

-

13

-

2

-

1

-

4

-

4

-

13

-

2

-

4

-

4

-

1

-

708650

Johnson

The ERAP2 gene is associated w ...

Homo sapiens

Hum. Genet.

126

655-666

2009

-

1

-

1

-

1

-

681600

Chen

Peptide hydrolysis by the binu ...

Vibrio proteolyticus

J. Phys. Chem. B

112

2494-2500

2008

-

1

-

1

-

1

-

700001

Schu

Aminopeptidase I enzymatic act ...

Saccharomyces cerevisiae

Methods Enzymol.

451

67-78

2008

-

2

1

-

2

-

2

1

-

2

1

-

2

1

-

677369

Adachi

Crystallization of Saccharomyc ...

Saccharomyces cerevisiae

Acta Crystallogr. Sect. F

63

200-203

2007

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

678993

Tishinov

-

Isolation and characterization ...

Helianthus annuus

C. R. Acad. Bulg. Sci.

60

1007-1010

2007

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

679310

Kostecka

-

Partial purification of larval ...

Musca domestica

Collect. Symp. Ser. Academy Sci. Czech Republic

9

66-68

2007

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

666422

Hammer

The aminopeptidase ERAAP shape ...

Mus musculus

Nat. Immunol.

7

103-112

2006

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

666760

York

Endoplasmic reticulum aminopep ...

Mus musculus

Proc. Natl. Acad. Sci. USA

103

9202-9207

2006

-

1

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

680333

Cortes

Purification of a novel aminop ...

Parietaria judaica

J. Allergy Clin. Immunol.

118

878-884

2006

-

13

1

-

1

-

1

-

1

-

1

2

-

9

-

1

-

1

-

1

-

1

-

13

-

1

-

1

-

1

-

1

2

-

9

-

1

-

1

-

1

-

1

-

665275

Nagata

Characteristics of an aminopep ...

Cryptomeria japonica

J. Agric. Food Chem.

53

5445-5448

2005

-

5

-

1

-

2

-

2

1

-

16

-

1

-

1

-

1

-

5

1

-

1

-

2

1

-

16

-

1

-

1

-

666737

Chang

The ER aminopeptidase, ERAP1, ...

Sus scrofa

Proc. Natl. Acad. Sci. USA

102

17107-17112

2005

-

13

1

-

1

-

1

-

1

-

2

1

-

22

-

1

-

1

-

13

1

-

1

-

1

-

2

1

-

22

-

1

-

1

-

663693

Huston

Purification, characterization ...

Colwellia psychrerythraea

Appl. Environ. Microbiol.

70

3321-3328

2004

1

-

1

-

5

3

1

2

1

-

4

-

1

-

6

1

2

-

4

3

1

-

1

-

1

-

5

-

3

1

2

1

-

1

-

6

1

2

-

4

3

1

-

665104

Caprioglio

-

Yeast aminopeptidases Ape2, Aa ...

Saccharomyces cerevisiae

Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds)

1

316-318

2004

-

1

-

7

-

3

1

3

1

-

1

-

2

1

-

6

1

-

1

-

1

-

7

-

3

1

3

1

-

2

1

-

6

1

-

1

-

665106

Suarez-Rendueles

-

Aminopeptidase I ...

Saccharomyces cerevisiae

Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds)

1

940-941

2004

-

1

-

1

-

2

-

1

-

2

-

2

-

1

-

1

-

1

-

2

-

2

-

2

-

1

-

665108

Motoshima

-

Bacillus aminopeptidase I ...

Geobacillus stearothermophilus

Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds)

1

968-970

2004

1

-

2

-

2

-

1

-

1

-

3

1

2

-

2

-

2

-

1

-

2

-

2

-

1

-

3

1

2

-

2

-

2

-

650891

Bozic

Partial purification and chara ...

Morimus funereus

Comp. Biochem. Physiol. B

134

231-241

2003

3

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

3

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

650924

Kuo

Overexpression, purification, ...

Geobacillus stearothermophilus, Geobacillus stearothermophilus NIB 8924

Curr. Microbiol.

47

40-45

2003

-

1

-

10

-

1

-

4

-

1

-

1

-

6

1

-

1

-

1

-

10

-

1

-

1

-

1

-

6

1

-

1

-

650142

Bienvenue

Hydrolysis of thionopeptides b ...

Vibrio proteolyticus

Biochemistry

41

3712-3719

2002

-

2

16

-

3

-

1

-

1

-

5

-

1

-

18

1

-

2

-

2

16

-

3

-

1

-

5

-

1

-

18

1

-

650364

Chien

Purification, characterization ...

Aspergillus sojae

Biochim. Biophys. Acta

1576

119-126

2002

-

1

-

2

-

1

2

-

1

-

1

-

1

-

8

2

1

2

-

1

-

1

-

2

-

1

2

-

1

-

1

-

8

2

1

2

-

1

-

653895

Desmarais

The 1.20 A resolution crystal ...

Vibrio proteolyticus

Structure

10

1063-1072

2002

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

665257

Noguchi

Isolation and characterization ...

Cryptomeria japonica

J. Agric. Food Chem.

50

3540-3543

2002

-

4

-

2

-

2

-

1

-

2

1

5

1

-

1

-

2

-

4

2

-

2

-

1

-

2

1

5

1

-

1

-

666143

Millership

Characterization of aminopepti ...

Encephalitozoon cuniculi, Encephalitozoon hellem

J. Parasitol.

88

843-848

2002

-

7

-

2

-

2

-

2

-

2

-

10

-

2

-

7

-

2

-

2

-

2

-

10

-

2

-

652119

Byun

Aminopeptidase from Sphingomon ...

Novosphingobium capsulatum

J. Biol. Chem.

276

17902-17907

2001

-

1

-

5

2

1

-

1

-

1

-

1

-

20

1

-

2

3

1

-

3

-

1

-

5

3

2

1

-

1

-

1

-

20

1

-

2

3

1

-

664347

Nishiwaki

Purification and characterizat ...

Grifola frondosa

Biosci. Biotechnol. Biochem.

65

424-427

2001

-

1

-

9

3

-

1

2

-

1

-

1

-

1

-

4

1

-

1

3

1

-

1

-

1

-

9

-

3

-

1

2

-

1

-

1

-

4

1

-

1

3

1

-

1

-

665255

Byun

Synergistic action of an X-pro ...

Aspergillus oryzae

J. Agric. Food Chem.

49

2061-2063

2001

-

2

-

2

-

1

-

1

-

2

-

1

-

1

-

665476

Andrei-Selmer

A new class of mutants deficie ...

Saccharomyces cerevisiae

J. Biol. Chem.

276

11606-11614

2001

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

650310

Cristofoletti

The role of amino acid residue ...

Tenebrio molitor

Biochim. Biophys. Acta

1479

185-195

2000

-

11

1

2

1

-

1

-

1

-

2

-

1

-

1

-

1

-

4

-

11

4

1

2

1

-

1

-

2

-

1

-

1

-

1

-

650315

Blinkovsky

A non-specific aminopeptidase ...

Aspergillus oryzae

Biochim. Biophys. Acta

1480

171-181

2000

-

1

-

3

7

1

2

-

4

-

1

-

2

-

12

1

-

2

18

1

-

1

-

3

-

7

1

2

-

1

-

2

-

12

1

-

2

18

1

-

665822

Desimone

Purification and characterizat ...

Hordeum vulgare

J. Chromatogr. B

737

285-293

2000

-

3

-

1

2

-

1

-

1

-

1

-

6

1

-

3

-

1

2

-

1

-

1

-

6

1

-

28283

Klionsky

Nonclassical protein sorting t ...

Saccharomyces cerevisiae

J. Biol. Chem.

273

10807-10810

1998

-

1

-

1

-

1

-

28284

Martinez

Folding of the presequence of ...

Saccharomyces cerevisiae

J. Mol. Biol.

267

1124-1138

1997

-

1

-

1

-

1

-

28285

Kim

Transport of a large oligomeri ...

Saccharomyces cerevisiae

J. Cell Biol.

137

609-618

1997

-

3

-

3

-

3

-

28286

Scott

Aminopeptidase I is targeted t ...

Saccharomyces cerevisiae

J. Cell Biol.

138

37-44

1997

-

3

-

2

-

3

-

28287

Oda

Identification of a cytoplasm ...

Saccharomyces cerevisiae

J. Cell Biol.

132

999-1010

1996

-

2

-

2

-

2

-

28288

Scott

In vitro reconstitution of cyt ...

Saccharomyces cerevisiae

J. Cell Biol.

3¦131

1727-1735

1995

-

1

-

2

-

1

-

28289

Segui-Real

Yeast aminopeptidase I is post ...

Saccharomyces cerevisiae

EMBO J.

14

5476-5484

1995

-

3

-

2

-

3

-

28290

Tisljar

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5

1

-

2

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2

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1

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1

13

1

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1

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5

-

1

-

2

-

1

-

1

13

1

-

1

-

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6

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2

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3

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2

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1

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1

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11

1

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2

-

6

-

2

-

3

-

1

-

1

-

11

1

-

2

-

28292

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2

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2

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1

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1

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3

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1

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1

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28296

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Saccharomyces cerevisiae

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1979

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1

-

2

-

2

-

28297

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The quarternary structure of y ...

Saccharomyces cerevisiae

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-

4

-

2

-

2

-

4

-

2

-

28298

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Yeast aminopeptidase I. Chemic ...

Saccharomyces cerevisiae

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7

-

2

-

2

-

1

-

2

1

14

1

-

1

2

-

1

-

7

-

2

-

1

-

2

1

14

1

-

1

2

-

1

-

Purification and characterization of the cystinyl bond cleaving yeast aminopeptidase yscXVI

Data extracted from this reference: