Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.22 extracted from

  • Tisljar, U.; Wolf, D.H.
    Purification and characterization of the cystinyl bond cleaving yeast aminopeptidase yscXVI (1993), FEBS Lett., 322, 191-196.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10 phenanthroline
-
Saccharomyces cerevisiae
amastatin
-
Saccharomyces cerevisiae
bestatin
-
Saccharomyces cerevisiae
EDTA
-
Saccharomyces cerevisiae
additional information no inhibition by phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, N.ethylmaleimide, pepstatin, phosphoramidon, puromycin Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0225
-
Cystinyl 4-nitroanilide
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ CoCl2 activates Saccharomyces cerevisiae
Zn2+ ZnCl2 has no influence on activity Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Saccharomyces cerevisiae
52000
-
gel filtration, non-denaturing PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no N-glycosylation Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
aminopeptidase yscXVI Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl buffer, pH 7.2, 38% loss of activity after 4 days, 81% loss of activity after 11 days Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Ala + 4-nitroaniline
-
?
Arg 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Arg + 4-nitroaniline
-
?
Cystinyl 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Cystine + 4-nitroaniline
-
?
Glu 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Glu + 4-nitroaniline
-
?
Gly 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Gly + 4-nitroaniline
-
?
Leu 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Leu + 4-nitroaniline
-
?
Lys 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Lys + 4-nitroaniline
-
?
Met 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Met + 4-nitroaniline
-
?
additional information no hydrolyis of succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, succinyl-Ala-Ala-Pro-Leu 4-nitroanilide, succinyl-Ala-Ala-Val- Ala 4-nitroanilide, benzyloxycarbonyl-Arg-Arg 4-nitroanilide, benzyloxycarbonyl-Gly-Pro 4-nitroanilide, benzyloxycarbonyl-Pro-Phe-Arg 4-nitroanilide, acetyl-Arg-Gly-Gly-Met 4-nitroanilide, benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide, benzoyl-Tyr 4-nitroanilide, benzyloxycarbonyl-Lys-Arg 4-nitroanilide Saccharomyces cerevisiae ?
-
?
Phe 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Phe + 4-nitroaniline
-
?
Pro 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Pro + 4-nitroaniline
-
?
S-Benzoyl-Cys 4-nitroanilide + H2O
-
Saccharomyces cerevisiae S-Benzoyl-Cys + 4-nitroaniline
-
?
Val 4-nitroanilide + H2O
-
Saccharomyces cerevisiae Val + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 substrate cystinyl 4-nitroanilide Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
4 9 no activity at pH 4 and pH 9, about 33% of maximal activity at pH 6 Saccharomyces cerevisiae