Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10 phenanthroline | - |
Saccharomyces cerevisiae | |
amastatin | - |
Saccharomyces cerevisiae | |
bestatin | - |
Saccharomyces cerevisiae | |
EDTA | - |
Saccharomyces cerevisiae | |
additional information | no inhibition by phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, N.ethylmaleimide, pepstatin, phosphoramidon, puromycin | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0225 | - |
Cystinyl 4-nitroanilide | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | CoCl2 activates | Saccharomyces cerevisiae | |
Zn2+ | ZnCl2 has no influence on activity | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Saccharomyces cerevisiae |
52000 | - |
gel filtration, non-denaturing PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | no N-glycosylation | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
aminopeptidase yscXVI | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl buffer, pH 7.2, 38% loss of activity after 4 days, 81% loss of activity after 11 days | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Ala + 4-nitroaniline | - |
? | |
Arg 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Arg + 4-nitroaniline | - |
? | |
Cystinyl 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Cystine + 4-nitroaniline | - |
? | |
Glu 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Glu + 4-nitroaniline | - |
? | |
Gly 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Gly + 4-nitroaniline | - |
? | |
Leu 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Leu + 4-nitroaniline | - |
? | |
Lys 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Lys + 4-nitroaniline | - |
? | |
Met 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Met + 4-nitroaniline | - |
? | |
additional information | no hydrolyis of succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, succinyl-Ala-Ala-Pro-Leu 4-nitroanilide, succinyl-Ala-Ala-Val- Ala 4-nitroanilide, benzyloxycarbonyl-Arg-Arg 4-nitroanilide, benzyloxycarbonyl-Gly-Pro 4-nitroanilide, benzyloxycarbonyl-Pro-Phe-Arg 4-nitroanilide, acetyl-Arg-Gly-Gly-Met 4-nitroanilide, benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide, benzoyl-Tyr 4-nitroanilide, benzyloxycarbonyl-Lys-Arg 4-nitroanilide | Saccharomyces cerevisiae | ? | - |
? | |
Phe 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Phe + 4-nitroaniline | - |
? | |
Pro 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Pro + 4-nitroaniline | - |
? | |
S-Benzoyl-Cys 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | S-Benzoyl-Cys + 4-nitroaniline | - |
? | |
Val 4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | Val + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | substrate cystinyl 4-nitroanilide | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | no activity at pH 4 and pH 9, about 33% of maximal activity at pH 6 | Saccharomyces cerevisiae |