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Literature summary for 3.4.11.21 extracted from

  • Watanabe, J.; Tanaka, H.; Akagawa, T.; Mogi, Y.; Yamazaki, T.
    Characterization of Aspergillus oryzae aspartyl aminopeptidase expressed in Escherichia coli (2007), Biosci. Biotechnol. Biochem., 71, 2557-2560.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 57% residual activity at 40 mM; 57% residual activity at 40 mM Aspergillus oryzae
EDTA 54% residual activity at 20 mM; 54% residual activity at 20 mM Aspergillus oryzae
NaCl about 30% of activity is retained in 20% (w/v) NaCl; about 30% of activity is retained in 20% (w/v) NaCl Aspergillus oryzae
o-phenanthroline 43% residual activity at 5 mM; 43% residual activity at 5 mM Aspergillus oryzae
Zn2+ 27% residual activity at 0.4 mM; 27% residual activity at 0.4 mM Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 294% increase of activity at 0.4 mM Aspergillus oryzae
Mn2+ 91% increase of activity at 0.4 mM Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54430
-
calculated from amino acid sequence Aspergillus oryzae
57700
-
SDS-PAGE Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q2U9Z9
-
-
Aspergillus oryzae Q2UPZ7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose 6 Fast Flow column chromatography Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin II + H2O
-
Aspergillus oryzae Asp + angiotensin III
-
?
Asp-p-nitroanilide + H2O 64% activity Aspergillus oryzae Asp + p-nitroaniline
-
?
Glu-p-nitroanilide + H2O 100% activity Aspergillus oryzae Glu + p-nitroaniline
-
?
additional information does not hydrolyze [Asn1, Val5]-angiotensin II, less than 0.5% activity on Ala-p-nitroanilide, Pro-p-nitroanilide, Met-p-nitroanilide, Arg-p-nitroanilide, and Leu-p-nitroanilide Aspergillus oryzae ?
-
?

Synonyms

Synonyms Comment Organism
angiotensinase
-
Aspergillus oryzae
AspAP
-
Aspergillus oryzae
aspartyl-AP
-
Aspergillus oryzae
DAP
-
Aspergillus oryzae
peptidase E
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme remains stabel upt to 50°C and retains about 70% activity after 20 h at 50°C, is inactivated rapidly at temperature in excess of this Aspergillus oryzae
50
-
the enzyme remains stable upt to 50°C and retains about 70% activity after 20 h at 50°C, is inactivated rapidly at temperature in excess of this Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Aspergillus oryzae