Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Mus musculus |
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His6-tagged enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition | Homo sapiens | |
1,10-phenanthroline | complete inhibition | Mus musculus | |
1,10-phenanthroline | complete inhibition | Oryctolagus cuniculus | |
DTT | high inhibition at 1 mM | Homo sapiens | |
DTT | high inhibition at 1 mM | Mus musculus | |
DTT | 88% inhibition at 1 mM | Oryctolagus cuniculus | |
EDTA | weak inhibition | Homo sapiens | |
EDTA | weak inhibition | Mus musculus | |
EDTA | weak inhibition | Oryctolagus cuniculus | |
additional information | no inhibition by Zn2+ in contrary to glutamyl aminopeptidase, EC 3.4.11.7, no inhibition by Glu-thiol, Asp-thiol, bestatin, amastatin, and puromycin | Homo sapiens | |
additional information | no inhibition by Zn2+ in contrary to glutamyl aminopeptidase, EC 3.4.11.7, no inhibition by Glu-thiol, Asp-thiol, bestatin, amastatin, and puromycin | Mus musculus | |
additional information | no inhibition by Zn2+ in contrary to glutamyl aminopeptidase, EC 3.4.11.7, no inhibition by Glu-thiol, Asp-thiol, bestatin, amastatin, and puromycin | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | - |
Oryctolagus cuniculus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by chloride ions | Mus musculus | |
additional information | the enzyme is not affected by chloride ions | Homo sapiens | |
additional information | the enzyme is not affected by chloride ions | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52428 | - |
8 * 55000, SDS-PAGE, 8 * 52428, amino acid sequence calculation | Homo sapiens |
55000 | - |
8 * 55000, SDS-PAGE, 8 * 52428, amino acid sequence calculation | Homo sapiens |
440000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity | Homo sapiens |
soluble enzyme from brain to homogeneity by affinity chromatography, gel filtration, hydrophobic interaction and hydroxyapatite chromatography | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate | the conserved residues His94, His170, and His440 are essential for activity | Mus musculus | |
release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate | the conserved residues His94, His170, and His440 are essential for activity | Homo sapiens | |
release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate | the conserved residues His94, His170, and His440 are essential for activity | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Oryctolagus cuniculus | - |
additional information | broad tissue distribution | Mus musculus | - |
additional information | broad tissue distribution | Homo sapiens | - |
additional information | broad tissue distribution | Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin II + H2O | i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | Mus musculus | Asp + angiotensin III | i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | ? | |
angiotensin II + H2O | i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | Homo sapiens | Asp + angiotensin III | i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | ? | |
angiotensin II + H2O | i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | Oryctolagus cuniculus | Asp + angiotensin III | i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | ? | |
Asp-Ala-Pro-chromogen + H2O | - |
Mus musculus | Asp + Ala-Pro-chromogen | - |
? | |
Asp-Ala-Pro-chromogen + H2O | - |
Homo sapiens | Asp + Ala-Pro-chromogen | - |
? | |
Asp-Ala-Pro-chromogen + H2O | - |
Oryctolagus cuniculus | Asp + Ala-Pro-chromogen | - |
? | |
Asp-Lys-Ala-Leu + H2O | lower activity | Mus musculus | Asp + Lys-Ala-Leu | - |
? | |
Asp-Lys-Ala-Leu + H2O | lower activity | Homo sapiens | Asp + Lys-Ala-Leu | - |
? | |
Asp-Lys-Ala-Leu + H2O | lower activity | Oryctolagus cuniculus | Asp + Lys-Ala-Leu | - |
? | |
additional information | substrate specificity, overview, the substrate chain length is important for activity, the enzyme has little or no activity towards aminoacyl-arylamines, no or little activity towards aspartyl dipeptides with a positively charged residue in the second position | Mus musculus | ? | - |
? | |
additional information | substrate specificity, overview, the substrate chain length is important for activity, the enzyme has little or no activity towards aminoacyl-arylamines, no or little activity towards aspartyl dipeptides with a positively charged residue in the second position | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, overview, the substrate chain length is important for activity, the enzyme has little or no activity towards aminoacyl-arylamines, no or little activity towards aspartyl dipeptides with a positively charged residue in the second position | Oryctolagus cuniculus | ? | - |
? | |
N-(alpha-L-aspartyl)-4-nitroanilide + H2O | - |
Mus musculus | L-aspartic acid + 4-nitroaniline | - |
? | |
N-(alpha-L-aspartyl)-4-nitroanilide + H2O | - |
Homo sapiens | L-aspartic acid + 4-nitroaniline | - |
? | |
N-(alpha-L-aspartyl)-4-nitroanilide + H2O | - |
Oryctolagus cuniculus | L-aspartic acid + 4-nitroaniline | - |
? | |
N-(alpha-L-glutamyl)-2-naphthylamide + H2O | - |
Mus musculus | L-glutamic acid + 2-naphthylamine | - |
? | |
N-(alpha-L-glutamyl)-2-naphthylamide + H2O | - |
Homo sapiens | L-glutamic acid + 2-naphthylamine | - |
? | |
N-(alpha-L-glutamyl)-2-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-glutamic acid + 2-naphthylamine | - |
? | |
N-(alpha-L-glutamyl)-4-nitroanilide + H2O | - |
Mus musculus | L-glutamic acid + 4-nitroaniline | - |
? | |
N-(alpha-L-glutamyl)-4-nitroanilide + H2O | - |
Homo sapiens | L-glutamic acid + 4-nitroaniline | - |
? | |
N-(alpha-L-glutamyl)-4-nitroanilide + H2O | - |
Oryctolagus cuniculus | L-glutamic acid + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 55000, SDS-PAGE, 8 * 52428, amino acid sequence calculation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DAP | - |
Mus musculus |
DAP | - |
Homo sapiens |
DAP | - |
Oryctolagus cuniculus |
More | the enzyme belongs to the peptidase family M18 | Mus musculus |
More | the enzyme belongs to the peptidase family M18 | Homo sapiens |
More | the enzyme belongs to the peptidase family M18 | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
neutral pH-optimum | Mus musculus |
additional information | - |
neutral pH-optimum | Homo sapiens |
additional information | - |
neutral pH-optimum | Oryctolagus cuniculus |
7.5 | - |
assay at | Mus musculus |
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Oryctolagus cuniculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | sequence calculation | - |
7.03 |