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Literature summary for 3.4.11.20 extracted from

  • Azimzadeh, O.; Sow, C.; Geze, M.; Nyalwidhe, J.; Florent, I.
    Plasmodium falciparum PfA-M1 aminopeptidase is trafficked via the parasitophorous vacuole and marginally delivered to the food vacuole (2010), Malar. J., 9, 189.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.20

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged p68 form expressed in Escherichia coli BL21(DE3) Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole parasitophorous Plasmodium falciparum 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum O96935
-
-

Purification (Commentary)

Purification (Comment) Organism
p68 form, immobilized metal ion affinity chromatography (Ni2+) Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-7-amido-4-methylcoumarin + H2O
-
 Plasmodium falciparum L-leucine + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
PfA-M1 aminopeptidase 3 different forms p120, p96, p68 Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 pure native enzyme, substrate L-Leu-7-amido-4-methylcoumarin Plasmodium falciparum

Expression

Organism Comment Expression
Plasmodium falciparum p120 form is expressed in ring stages at least 12 hours before p68 is detected up

General Information

General Information Comment Organism
physiological function synthesized as the precursor p120 form, targeted to the parasitophorous vacuole via the parasite endoplasmic reticulum/Golgi, where it is converted into the transient p96 form; p96 form is eventually redirected into the parasite to be converted into the processed p68 form that is only marginally delivered to the parasite foodvacuole Plasmodium falciparum