Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition at 1 mM | Rattus norvegicus | |
amastatin | 0.1 mM, 1% residual activity; 99% inhibiton at 0.1 mM | Rattus norvegicus | |
bestatin | 0.1 mM, 7% residual activity; 93% inhibiton at 0.1 mM | Rattus norvegicus | |
Co2+ | 58% inhibition at 1 mM | Rattus norvegicus | |
copper | 1 mM, 5% resiudal activity | Rattus norvegicus | |
Cu2+ | 95% inhibition at 1 mM | Rattus norvegicus | |
EDTA | 23% inhibition at 5 mM; 5 mM, 77% residual activity | Rattus norvegicus | |
EGTA | 15% inhibition at 5 mM; 5 mM, 85% residual activity | Rattus norvegicus | |
Mn2+ | 44% inhibition at 1 mM | Rattus norvegicus | |
Ni2+ | 71% inhibition at 1 mM | Rattus norvegicus | |
o-phenanthroline | 1 mM, no residual activity | Rattus norvegicus | |
Zinc | 1 mM, 1% resiudal activity | Rattus norvegicus | |
Zn2+ | 99% inhibition at 1 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border | - |
Rattus norvegicus | 5903 | - |
soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no or poor effects by 1 mM of Mg2+ and Ca2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P15684 | - |
- |
Rattus norvegicus Wistar | P15684 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
native enzyme partially from rat kidney by gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bile canaliculus | surface membrane | Rattus norvegicus | - |
hepatocyte | positive staining for the enzyme is limited strictly to the membrane of the hepatocytes that consist of bile canaliculi | Rattus norvegicus | - |
kidney | located in brush border of a renal proximal tubule and the small intestine, and the surface membrane of bile canaliculi | Rattus norvegicus | - |
kidney | renal proximal tubule, no immunoreactivity to the antiserum is found in the glomeruli, in the distal renal tubular epithelial cells, or in the renal tubules in the medulla | Rattus norvegicus | - |
liver | located in brush border of a renal proximal tubule and the small intestine, and the surface membrane of bile canaliculi | Rattus norvegicus | - |
liver | positive staining for the enzyme is limited strictly to the membrane of the hepatocytes that consist of bile canaliculi, but no signal is seen in the sinusoidal wall. The low expression of the enzyme in the liver might be due to the limited localization on the bile canaliculi | Rattus norvegicus | - |
additional information | immunohistochemic analysis | Rattus norvegicus | - |
renal proximal tubule | - |
Rattus norvegicus | - |
small intestine | brush border of the epithelium in the small intestine | Rattus norvegicus | - |
small intestine | located in brush border of a renal proximal tubule and the small intestine, and the surface membrane of bile canaliculi | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-7-amido-4-carbamoylmethylcoumarin + H2O | - |
Rattus norvegicus | L-Ala + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-Ala-7-amido-4-carbamoylmethylcoumarin + H2O | - |
Rattus norvegicus Wistar | L-Ala + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-alanine 4-methylcoumaryl-7-amide + H2O | Ala-MCA | Rattus norvegicus | L-alanine + 7-amino-4-methylcoumarin | - |
? | |
L-alanyl-L-alanyl-L-7-amido-4-carbamoylmethylcoumarin + H2O | substrate for use with tripeptidyl peptidases | Rattus norvegicus | L-alanyl-L-alanine + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-alanyl-L-alanyl-L-phenylalanine 4-methylcoumaryl-7-amide + H2O | Ala-Ala-Phe-MCA or AAF-MCA | Rattus norvegicus | L-alanyl-L-alanyl-L-phenylalanine + 7-amino-4-methylcoumarin | - |
? | |
L-arginine 4-methylcoumaryl-7-amide + H2O | Arg-MCA | Rattus norvegicus | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-carbamoylmethylcoumarin + H2O | 70% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin | Rattus norvegicus | L-Leu + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-Leu-7-amido-4-carbamoylmethylcoumarin + H2O | 70% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin | Rattus norvegicus Wistar | L-Leu + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-leucine 4-methylcoumaryl-7-amide + H2O | Leu-MCA | Rattus norvegicus | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
L-lysine 4-methylcoumaryl-7-amide + H2O | Lys-MCA, low activity | Rattus norvegicus | L-lysine + 7-amino-4-methylcoumarin | - |
? | |
L-Met-7-amido-4-carbamoylmethylcoumarin + H2O | 99% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin | Rattus norvegicus | L-Met + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-Met-7-amido-4-carbamoylmethylcoumarin + H2O | 99% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin | Rattus norvegicus Wistar | L-Met + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-methionine 4-methylcoumaryl-7-amide + H2O | Met-MCA | Rattus norvegicus | L-methionine + 7-amino-4-methylcoumarin | - |
? | |
L-Phe-7-amido-4-carbamoylmethylcoumarin + H2O | 76% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin | Rattus norvegicus | L-Phe + 7-amino-4-carbamoylmethylcoumarin | - |
? | |
L-phenylalanine 4-methylcoumaryl-7-amide + H2O | Phe-MCA | Rattus norvegicus | L-phenylalanine + 7-amino-4-methylcoumarin | - |
? | |
additional information | enzyme preferentially cleaves neutral and nonpolar amino acids such as alanine, methionine, leucine, and phenylalanine at the P1 position, substrates with basic amino acids such as lysine and arginine at the P1 position are less cleaved. Pyroglutamic acid, and Bz-Arginine, are not cleaved at all | Rattus norvegicus | ? | - |
? | |
additional information | the peptidase that hydrolyzes AAF-MCA is the soluble form of aminopeptidase N/CD13, and caution is required when using AAF-MCA as a substrate for tripeptidyl peptidase assays. No activity with Pyr-MCA and Bz-Arg-MCA | Rattus norvegicus | ? | - |
? | |
additional information | enzyme preferentially cleaves neutral and nonpolar amino acids such as alanine, methionine, leucine, and phenylalanine at the P1 position, substrates with basic amino acids such as lysine and arginine at the P1 position are less cleaved. Pyroglutamic acid, and Bz-Arginine, are not cleaved at all | Rattus norvegicus Wistar | ? | - |
? | |
additional information | the peptidase that hydrolyzes AAF-MCA is the soluble form of aminopeptidase N/CD13, and caution is required when using AAF-MCA as a substrate for tripeptidyl peptidase assays. No activity with Pyr-MCA and Bz-Arg-MCA | Rattus norvegicus Wistar | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 137000, SDS-PAGE | Rattus norvegicus |
? | x * 137000, enzyme from kidney, SDS-PAGE, x * 110000, enzyme from small intestine, SDS-PAGE, x * 140000, enzyme from liver, SDS-PAGE | Rattus norvegicus |
More | the N-terminal amino acid sequence of the peptidase is x-Ala-Pro-x-Leu-Pro-Gly-Ser-Thr-Ser-Ala-Thr-x-x-Ser, where x indicates undetectable amino acid residues. The undetectable amino acid residues such as threonine or serine might be modified residues that often undergo glycosylation or phosphorylation | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
alanyl aminopeptidase | - |
Rattus norvegicus |
aminopeptidase N | - |
Rattus norvegicus |
ANPEP | - |
Rattus norvegicus |
CD13 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |