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Literature summary for 3.4.11.2 extracted from

  • Vanderheyden, P.M.; Demaegdt, H.; Swales, J.; Lenaerts, P.J.; De Backer, J.P.; Vogel, L.K.; Vauquelin, G.
    Synergistic inhibition of the enzymatic activity of aminopeptidase N by divalent metal ion chelators (2006), Fundam. Clin. Pharmacol., 20, 613-619.
    View publication on PubMed
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cell Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Homo sapiens
1,10-phenanthroline
-
 Sus scrofa
EDTA
-
 Homo sapiens
EDTA
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 L-Leu-4-nitroanilide pH 7.4, 37°C Sus scrofa
0.25
-
 L-Leu-4-nitroanilide pH 7.4, 37°C, presence of 0.15 mM EDTA Sus scrofa
0.43
-
 L-Leu-4-nitroanilide pH 7.4, 37°C Homo sapiens
0.6
-
 L-Leu-4-nitroanilide pH 7.4, 37°C, presence of 0.15 mM EDTA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-
soluble
-
 Sus scrofa
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
 commercial preparation
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
 Homo sapiens L-Leu + 4-nitroanilide
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Sus scrofa L-Leu + 4-nitroanilide
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.012
-
 pH 7.4, 37°C, presence of 0.05 mM EDTA Homo sapiens 1,10-phenanthroline
0.027
-
 pH 7.4, 37°C, presence of 0.005 mM EDTA Homo sapiens 1,10-phenanthroline
0.046
-
 pH 7.4, 37°C, presence of 0.05 mM EDTA Sus scrofa 1,10-phenanthroline
0.119
-
 pH 7.4, 37°C, presence of 0.005 mM EDTA Sus scrofa 1,10-phenanthroline
0.178
-
 pH 7.4, 37°C Sus scrofa 1,10-phenanthroline
0.179
-
 pH 7.4, 37°C Homo sapiens 1,10-phenanthroline