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Literature summary for 3.4.11.2 extracted from

  • Jamadar, V.K.; Jamdar, S.N.; Dandekar, S.P.; Harikumar, P.
    Purification and characterization of aminopeptidase from chicken intestine (2003), J. Food Sci., 68, 438-443.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strong inhibition Gallus gallus
bestatin strong inhibition Gallus gallus
puromycin strong inhibition Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
1 * 94000 + 1 * 66000, SDS-PAGE Gallus gallus
94000
-
1 * 94000 + 1 * 66000, SDS-PAGE Gallus gallus
150000
-
about, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 839fold from intestine to homogeneity by ion exchange chromatography, gel filtration, hydrophobic interaction chromatography, and affinity chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-2-naphthylamide + H2O best substrate Gallus gallus L-Ala + 2-naphthylamine
-
?
L-Arg-2-naphthylamide + H2O
-
Gallus gallus L-Arg + 2-naphthylamine
-
?
L-Leu-2-naphthylamide + H2O
-
Gallus gallus L-Leu + 2-naphthylamine
-
?
additional information substrate specificity, overview Gallus gallus ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 94000 + 1 * 66000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Gallus gallus