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Literature summary for 3.4.11.2 extracted from
- A mutation in aminopeptidase N (CD13) isolated from a patient suffering from leukemia leads to an arrest in the endoplasmic reticulum (2006), J. Biol. Chem., 281, 11894-11900.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | the enzyme is used as a routine marker for myelomonocytic cells in hematopoietic malignant disorders | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient expression of wild-type and mutant enzymes in COS-1 cells, as GFP-tagged proteins, and in renal carcinoma N109 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L243P | a natural mutation in the enzyme isolated from a patient suffering from leukemia leads to an arrest in the endoplasmic reticulum, the mutant is inactive at physiological temperatures of 32-37°C, persists as a mannose-rich proteinin the endoplasmic reticulum, and is degraded by a ER-associated degradation pathway, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | wild-type mature enzyme | Homo sapiens | 9986 | - |
| endoplasmic reticulum | mannose-rich precursor enzyme form | Homo sapiens | 5783 | - |
| Golgi apparatus | wild-type mature enzyme | Homo sapiens | 5794 | - |
| additional information | intracellular distribution pattern | Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 166000 | - |
1 * 170000, mannose-rich precursor, SDS-PAGE, 1 * 166000, complex glycosylated mature enzyme, SDS-PAGE, 1 * 190000, about, recombinant GFP-tagged complex glycosylated mature enzyme, SDS-PAGE | Homo sapiens |
| 170000 | - |
1 * 170000, mannose-rich precursor, SDS-PAGE, 1 * 166000, complex glycosylated mature enzyme, SDS-PAGE, 1 * 190000, about, recombinant GFP-tagged complex glycosylated mature enzyme, SDS-PAGE | Homo sapiens |
| 190000 | - |
1 * 170000, mannose-rich precursor, SDS-PAGE, 1 * 166000, complex glycosylated mature enzyme, SDS-PAGE, 1 * 190000, about, recombinant GFP-tagged complex glycosylated mature enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme might contribute to extracellular matrix degradation, the enzyme shows increased surface expression in cases of malignant transformation, inflamation, or T-cell activation | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
leukemic patient | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | mannose-rich precursor, complex glycosylated mature enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | expression pattern, the enzyme shows increased surface expression in cases of malignant transformation, inflammation, or T-cell activation, no activity in B-cells and resting T-cells | Homo sapiens | - |
| T-cell | activated | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala-4-nitroanilide + H2O | - |
Homo sapiens | L-Ala + 4-nitroaniline | - |
? | |
| additional information | the enzyme might contribute to extracellular matrix degradation, the enzyme shows increased surface expression in cases of malignant transformation, inflamation, or T-cell activation | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 170000, mannose-rich precursor, SDS-PAGE, 1 * 166000, complex glycosylated mature enzyme, SDS-PAGE, 1 * 190000, about, recombinant GFP-tagged complex glycosylated mature enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase N | - |
Homo sapiens |
| APN | - |
Homo sapiens |
| CD13 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | 37 | assay at | Homo sapiens |
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