Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.2 extracted from

  • Lendeckel, U.; Bukowska, A.; Laettig, J.H.; Brandt, W.
    Alanyl-aminopeptidases in human T cells. Structures and functions (2004), Aminopeptidases in Biology and Disease (Hooper, N. M. ; Lendechel, U. , eds. ), 2, 201-227.
No PubMed abstract available
Search for more literature for 3.4.11.2

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is induced by interleukin-4, IFNgamma, CD3, CD28, and concanavalin A Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression analysis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
actinonin IC50: 0.000075 mM, kidney enzyme Homo sapiens
bestatin inhibits enzyme activity and cell growth, e.g. of leukocytes, overview, IC50: 0.0004 mM, kidney enzyme Homo sapiens
bradykinin inhibition is enhanced by substance P Homo sapiens
iodoacetamide IC50: 0.098 mM, kidney enzyme Homo sapiens
additional information structural features of natural peptide hormone inhibitors, overview Homo sapiens
morphiceptin
-
 Homo sapiens
PAQ-22 IC50: 0.00029 mM, kidney enzyme Homo sapiens
phebestin IC50: 0.000015 mM, kidney enzyme Homo sapiens
probestin IC50: 0.000016 mM, kidney enzyme Homo sapiens
puromycin IC50: 0.0031 mM, kidney enzyme Homo sapiens
ranatachykinin
-
 Homo sapiens
RB3014 IC50: 0.000025 mM, kidney enzyme Homo sapiens
Substance P
-
 Homo sapiens
substance P1-4
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface ectoenzyme, the enzyme has an N-terminal membrane anchor Homo sapiens 9986
-
membrane type II transmembrane protein expressed on the cell suface Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-metallopeptidase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens mechanism of APN signaling, enzyme inhibition has anti-proliferative effect and causes strong immunosuppression both in vitro and in vivo including growth arrest, induction of immunosuppressive cytokines, e.g. TGF-beta1, reduced expression of inflammatory or T-cell stimulating cytokines, e.g. interleukin-2 and interleukin-12, and mosulation of T-cell signalling pathways, overview, catalytic mechanism, ligand binding structure, active site structure, dynamic opening and closing ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
B-cell leukemia cell
-
 Homo sapiens
-
epithelial cell
-
 Homo sapiens
-
granulocyte
-
 Homo sapiens
-
H9 cell
-
 Homo sapiens
-
intestine
-
 Homo sapiens
-
K-562 cell
-
 Homo sapiens
-
KARPAS-299 cell
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
leukocyte
-
 Homo sapiens
-
macrophage
-
 Homo sapiens
-
monocyte
-
 Homo sapiens
-
additional information wide tissue distribution, expression analysis, detailed overview Homo sapiens
-
myelocytic leukemia cell
-
 Homo sapiens
-
P-12 cell
-
 Homo sapiens
-
pericardial fluid
-
 Homo sapiens
-
synovial fluid
-
 Homo sapiens
-
T-lymphocyte activated Homo sapiens
-
THP-1 cell
-
 Homo sapiens
-
thymocyte
-
 Homo sapiens
-
U-937 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O
-
 Homo sapiens L-Ala + 4-nitroaniline
-
 ?
additional information mechanism of APN signaling, enzyme inhibition has anti-proliferative effect and causes strong immunosuppression both in vitro and in vivo including growth arrest, induction of immunosuppressive cytokines, e.g. TGF-beta1, reduced expression of inflammatory or T-cell stimulating cytokines, e.g. interleukin-2 and interleukin-12, and mosulation of T-cell signalling pathways, overview, catalytic mechanism, ligand binding structure, active site structure, dynamic opening and closing Homo sapiens ?
-
 ?
additional information enzyme inhibition causes strong immunosuppression both in vitro and in vivo Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer monomer structure, three-dimensional sructure, and structure modelling using the crystal structure, dynamic opening and closing of the active site Homo sapiens

Synonyms

Synonyms Comment Organism
alanyl-aminopeptidase
-
 Homo sapiens
aminopeptidase N
-
 Homo sapiens
APN
-
 Homo sapiens
CD13
-
 Homo sapiens
mAAP
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00044
-
 Substance P
-
 Homo sapiens
0.0094
-
 bradykinin
-
 Homo sapiens
0.169
-
 morphiceptin
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000015
-
 IC50: 0.000015 mM, kidney enzyme Homo sapiens phebestin
0.000016
-
 IC50: 0.000016 mM, kidney enzyme Homo sapiens probestin
0.000025
-
 IC50: 0.000025 mM, kidney enzyme Homo sapiens RB3014
0.000075
-
 IC50: 0.000075 mM, kidney enzyme Homo sapiens actinonin
0.00029
-
 IC50: 0.00029 mM, kidney enzyme Homo sapiens PAQ-22
0.0004
-
 inhibits enzyme activity and cell growth, e.g. of leukocytes, overview, IC50: 0.0004 mM, kidney enzyme Homo sapiens bestatin
0.0031
-
 IC50: 0.0031 mM, kidney enzyme Homo sapiens puromycin
0.098
-
 IC50: 0.098 mM, kidney enzyme Homo sapiens iodoacetamide