Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Escherichia coli | |
4-chloromercuribenzoate | - |
Escherichia coli | |
amastatin | - |
Escherichia coli | |
bestatin | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
puromycin | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Leu-2-naphthylamide | - |
Escherichia coli | |
0.74 | - |
Gly-2-naphthylamide | - |
Escherichia coli | |
1 | - |
Ala-2-naphthylamide | - |
Escherichia coli | |
2.86 | - |
Ala-p-nitroanilide | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | reactivation after inhibition by 1,10-phenanthroline | Escherichia coli | |
Zn2+ | at concentrations between 0.1 and 0.3 mM reactivation after inhibition by 1,10-phenanthroline, inhibitory at higher concentrations | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aminoacyl peptide | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.78 | - |
Gly-2-naphthylamide as substrate | Escherichia coli |
1.6 | - |
Met-2-naphthylamide as substrate | Escherichia coli |
1.68 | - |
Ala-p-nitroanilide as substrate | Escherichia coli |
3.54 | - |
Ala-2-naphthylamide as substrate | Escherichia coli |
4.8 | - |
Leu-2-naphthylamide as substrate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-2-naphthylamide + H2O | - |
Escherichia coli | Ala + 2-naphthylamine | - |
? | |
Ala-p-nitroanilide + H2O | - |
Escherichia coli | Ala + p-nitroaniline | - |
? | |
aminoacyl peptide | - |
Escherichia coli | ? | - |
? | |
dipeptides + H2O | not: Leu-Pro | Escherichia coli | amino acids | - |
? | |
Gly-2-naphthylamide + H2O | - |
Escherichia coli | Gly + 2-naphthylamine | - |
? | |
Leu-2-naphthylamide + H2O | - |
Escherichia coli | Leu + 2-naphthylamine | - |
? | |
Leu-Gly + H2O | - |
Escherichia coli | Leu + Gly | - |
? | |
Met-2-naphthylamide + H2O | - |
Escherichia coli | Met + 2-naphthylamine | - |
? | |
tripeptides + H2O | not: Gly-Pro-Ala | Escherichia coli | dipeptides + amino acids | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
activity was measured for 5 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | stable for 1h at 30°C | Escherichia coli |