Any feedback?
Literature summary for 3.4.11.2 extracted from
- Characterization of aminopeptidase N from the brush border membrane of the larvae midgut of silkworm, Bombyx mori as a zinc enzyme [published erratum appears in Biochim Biophys Acta 1998 Sep 8;1387(1-2):486 (1998), Biochim. Biophys. Acta, 1383, 301-310.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 97% inhibition after 10 min at 10 mM | Bombyx mori |  |
| actinonin | IC50: 148 microM | Bombyx mori | |
| amastatin | IC50: 7.6 microM | Bombyx mori | |
| bestatin | poor inhibitor, IC50: 3.25 mM | Bombyx mori | |
| Cu2+ | - |
Bombyx mori | |
| EDTA | only 27% inhibition at 300 mM, 50% inhibition after incubation for 12 h with 300 mM | Bombyx mori | |
| probestin | IC50: 74 microM | Bombyx mori | |
| Zn2+ | inhibits at concentrations above 0.1 mM | Bombyx mori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.222 | - |
Leu-4-nitroanilide | - |
Bombyx mori | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | brush border membrane | Bombyx mori | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | increase of activity | Bombyx mori | |
| Mg2+ | increase of activity | Bombyx mori | |
| Zn2+ | increase of activity at concentrations below 0.1 mM | Bombyx mori | |
| Zn2+ | content: 0.72 mol/mol protein | Bombyx mori | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aminoacyl peptide | Bombyx mori | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | larvae | Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aminoacyl peptide | - |
Bombyx mori | ? | - |
? | |
| Leu-p-nitroanilide + H2O | - |
Bombyx mori | Leu + p-nitroaniline | - |
? | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0076 | - |
IC50: 7.6 microM | Bombyx mori | amastatin | |
| 0.074 | - |
IC50: 74 microM | Bombyx mori | probestin | |
| 0.148 | - |
IC50: 148 microM | Bombyx mori | actinonin | |
| 3.25 | - |
poor inhibitor, IC50: 3.25 mM | Bombyx mori | bestatin | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
