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Literature summary for 3.4.11.2 extracted from

  • Huang, K.; Takahara, S.; Kinouchi, T.; Takeyama, M.; Ishida, T.; Ueyama, H.; Nishi, K.; Ohkubo, I.
    Alanyl aminopeptidase from human seminal plasma: purification, characterization, and immunohistochemical localization in the male genital tract (1997), J. Biochem., 122, 779-787.
    View publication on PubMed

General Stability

General Stability Organism
resistant against several cycles of freezing and thawing Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 100% inhibition at 1 mM Homo sapiens
actinonin 100% inhibition at 0.1 mM Homo sapiens
amastatin 100% inhibition at 0.1 mM Homo sapiens
arphamenine A 42% inhibition at 0.5 mM Homo sapiens
bestatin 96% inhibition at 0.1 mM Homo sapiens
Cd2+ 98% inhibition at 1 mM Homo sapiens
Co2+ 40% inhibition at 1 mM Homo sapiens
Cu2+ 96% inhibition at 1 mM Homo sapiens
EDTA 42% inhibition at 2 mM Homo sapiens
Hg2+ 71% inhibition at 1 mM Homo sapiens
leuhistin 100% inhibition at 0.1 mM Homo sapiens
Mg2+ not inhibitory at 1 mM Homo sapiens
Mn2+ 16% inhibition at 1 mM Homo sapiens
puromycin 67% inhibition at 0.1 mM Homo sapiens
Zn2+ 96% inhibition at 1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1 0.33 amino acyl-4-methylcoumarin 7-amide Ala-, Met-, Tyr-, Leu-, Phe-, Arg-, Lys-, Gly-derivatives Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
137000 153000 light scattering, native PAGE, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aminoacyl peptide Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
Homo sapiens
-
seminal plasma
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-30óC, at least four months, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aminoacyl peptide
-
Homo sapiens ?
-
?
aminoacyl-4-methylcoumarin 7-amide + H2O Ala-, Met-, Tyr-, Leu-, Phe-, Arg-, Lys-, Gly-, Lys-Ala-, Arg-Arg- derivatives, not: Pro-, Pyr-, Gly-Pro-derivatives Homo sapiens amino acid + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 137000-153000, light scattering, native PAGE, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable for at least 30 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 stable Homo sapiens