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Literature summary for 3.4.11.19 extracted from

  • Marui, J.; Matsushita-Morita, M.; Tada, S.; Hattori, R.; Suzuki, S.; Amano, H.; Ishida, H.; Yamagata, Y.; Takeuchi, M.; Kusumoto, K.
    Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji) (2012), Appl. Microbiol. Biotechnol., 93, 655-669 .
    View publication on PubMed
Search for more literature for 3.4.11.19

Inhibitors

Inhibitors Comment Organism Structure
AEBSF
-
 Aspergillus oryzae
benzamidine
-
 Aspergillus oryzae
additional information not inhibitory: bestatin Aspergillus oryzae
PCMB
-
 Aspergillus oryzae
TLCK
-
 Aspergillus oryzae
TPCK
-
 Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
126300
-
 gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae G7H7Y1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified GdaA might be digested during the final step of purification without affecting the activity and dimerization under nondenaturing conditions Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala 4-nitroanilide + H2O 93% of the activity with Gly 4-nitroanilide Aspergillus oryzae D-Ala + 4-nitroaniline
-
 ?
D-Ala-D-Ala + H2O
-
 Aspergillus oryzae 2 D-Ala
-
 ?
D-Ala-Gly + H2O
-
 Aspergillus oryzae D-Ala + Gly
-
 ?
D-Ala-Gly-Gly + H2O
-
 Aspergillus oryzae D-Ala + Gly-Gly
-
 ?
D-Ala-L-Ala + H2O
-
 Aspergillus oryzae D-Ala + L-Ala
-
 ?
Gly 4-nitroanilide + H2O
-
 Aspergillus oryzae Gly + 4-nitroaniline
-
 ?
Gly-Gly + H2O
-
 Aspergillus oryzae Gly + Gly
-
 ?
Gly-Gly-Gly + 2 H2O
-
 Aspergillus oryzae 3 Gly
-
 ?
Gly-L-Ala + H2O
-
 Aspergillus oryzae Gly + L-Ala
-
 ?
Gly-L-Cys + H2O
-
 Aspergillus oryzae Gly + L-Cys
-
 ?
Gly-L-Cys-Gly + H2O
-
 Aspergillus oryzae Gly + L-Cys-Gly
-
 ?
L-Ala 4-nitroanilide + H2O 12% of the activity with Gly 4-nitroanilide Aspergillus oryzae L-Ala + 4-nitroaniline
-
 ?
additional information substrates beta-Ala-4-nitroanilide, L-Leu-4-nitroanilide, D-Leu-4-nitroanilide, L-Met-4-nitroanilide, L-Pro-4-nitroanilide, L-Val-4-nitroanilide, L-Ile-4-nitroanilide, L-Phe-4-nitroanilide, D-Phe-4-nitroanilide, L-Lys-4-nitroanilide, L-Arg-4-nitroanilide, L-His-4-nitroanilide, L-Asp-4-nitroanilide, L-Glu-4-nitroanilide show less than 0.5% of the activity with Gly-4-nitroanilide, no substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide Aspergillus oryzae ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 59300, calculated from sequence Aspergillus oryzae

Synonyms

Synonyms Comment Organism
GdaA
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
5 11
-
 Aspergillus oryzae

General Information

General Information Comment Organism
physiological function GdaA is largely responsible for intracellular glycine and D-alanine aminopeptidase activities in Aspergillus oryzae during stationary-phase growth in liquid media. In addition, the activity increases in response to the depletion of nitrogen or carbon sources in the growth media. No significant differences are observed in the vegetative growth and conidiation on minimal and complete media in the GdaA deletion or overexpression strains, and control strains Aspergillus oryzae