Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterococcus faecalis |
Crystallization (Comment) | Organism |
---|---|
apo-form of enzyme and modeling of inhibitor 2,2'-bipyridine. One of the rings of 2,2'-bipyridine that points into the S1 pocket is surrounded by residues Tyr60, Tyr63, Trp218, and Cys68, while the other ring points towards the surface of the protein that does not make any contact with the protein, except with the metal ion | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridine | maximum inhibition is observed in the presence of CoCl2 rather than other metal ions | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000247 | - |
L-methionine 4-nitroanilide | pH 7.5, 30°C | Enterococcus faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | absolutely required, maximal enzyme activity is observed at 3.0 molar equivalents | Enterococcus faecalis | |
Mn2+ | may substitute for Co2+, about 50% of cobalt activation | Enterococcus faecalis | |
Ni2+ | may substitute for Co2+, maximal enzyme activity is observed at 10.0 molar equivalents | Enterococcus faecalis | |
Zn2+ | may substitute for Co2+, maximal enzyme activity is observed at 10.0 molar equivalents. Activity with Zn2+ is not dependent on pH | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine 4-nitroanilide + H2O | - |
Enterococcus faecalis | L-Met + 4-nitroaniline | - |
? | |
additional information | no substrates: Gly 4-nitroanilide , Ala 4-nitroanilide, Ser 7-amido-4-methyl-coumarin, Leu 4-nitroanilide, Glu 4-nitroanilide, Lys 4-nitroanilide and Arg 4-nitroanilide | Enterococcus faecalis | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
L-methionine 4-nitroanilide | pH 7.5, 30°C | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
highest activity in presence of Co2+, Mn2+, Ni2+ | Enterococcus faecalis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000667 | - |
pH 7.5, 30°C | Enterococcus faecalis | 2,2'-bipyridine |