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Literature summary for 3.4.11.18 extracted from

  • Chiu, J.; Wong, J.W.; Hogg, P.J.
    Redox regulation of methionine aminopeptidase 2 activity (2014), J. Biol. Chem., 289, 15035-15043.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.18

Organism

Organism UniProt Comment Textmining
Homo sapiens P50579 isoform MetAP2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information isoform MetAP2 is post-translationally regulated by an allosteric disulfide bond, which controls substrate specificity and catalytic efficiency. Isoform MetAP2 contains a single Cys228-Cys448 disulfide bond that has an -RH-staple configuration and links two beta-loop structures, which are hallmarks of allosteric disulfide bonds. The disulfide bond exists in oxidized and reduced states in the recombinant enzyme. The disulfide has a standard redox potential of -261 mV and is efficiently reduced by the protein reductant, thioredoxin, with a rate constant of 16180 per M and s. The MetAP2 disulfide bond also exists in oxidized and reduced states in glioblastoma tumor cells, and stressing the cells by oxygen or glucose deprivation results in more oxidized enzyme. Oxidized and reduced isoforms have different catalytic efficiencies for hydrolysis of MetAP2 peptide substrates Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
 substrate MVKQIESK , oxidized enzyme, pH 7.2, 25°C Homo sapiens
2.8
-
 substrate MVNPTVFF, oxidized enzyme, pH 7.2, 25°C Homo sapiens
3.1
-
 substrate MVNPTVFF, reduced enzyme, pH 7.2, 25°C Homo sapiens
3.4
-
 substrate MGNLKSVA, oxidized enzyme, pH 7.2, 25°C Homo sapiens
4.7
-
 substrate MVKQIESK , reduced enzyme, pH 7.2, 25°C Homo sapiens
7.8
-
 substrate MGNLKSVA, reduced enzyme, pH 7.2, 25°C Homo sapiens
15
-
 substrate MGKVKVGV, oxidized enzyme, pH 7.2, 25°C Homo sapiens
18
-
 substrate MGKVKVGV, reduced enzyme, pH 7.2, 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
MGKVKVGV + H2O N-terminal peptide of glyceraldehyde 3-phosphate dehydrogenase Homo sapiens L-Met + GKVKVGV
-
 ?
MGNLKSVA + H2O N-terminal peptide of endothelial nitric oxide synthase Homo sapiens L-Met + GNLKSVA
-
 ?
MVKQIESK + H2O N-terminal peptide of thioredoxin-1 Homo sapiens L-Met + VKQIESK
-
 ?
MVNPTVFF + H2O N-terminal peptide of cyclophilin A Homo sapiens L-Met + VNPTVFF
-
 ?

Synonyms

Synonyms Comment Organism
MetAP2
-
 Homo sapiens