Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | complete inhibition at 10 mM | Mycolicibacterium smegmatis | |
actinonin | 12.6% relative activity at 0.05 mM | Mycolicibacterium smegmatis | |
amastatin | 29.7% relative activity at 0.05 mM | Mycolicibacterium smegmatis | |
bestatin | 44.9% relative activity at 0.05 mM | Mycolicibacterium smegmatis | |
Cu2+ | - |
Mycolicibacterium smegmatis | |
EDTA | 33.7% relative activity at 100 mM, the enzyme activity inhibited by 1 mM EDTA is restored in presence of 5 mM Mg2+ | Mycolicibacterium smegmatis | |
Fe2+ | - |
Mycolicibacterium smegmatis | |
K+ | - |
Mycolicibacterium smegmatis | |
phenylmethylsulfonyl fluoride | 29.4% relative activity at 5 mM | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme activity is enhanced by 1 mM Co2+ | Mycolicibacterium smegmatis | |
Mg2+ | the enzyme activity is enhanced 2fold by 1 mM Mg2+ | Mycolicibacterium smegmatis | |
Na+ | some positive effect on the enzyme activity at 5 mM | Mycolicibacterium smegmatis | |
NH4+ | some positive effect on the enzyme activity at 5 mM | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
the calculated molecular weight from nucleotide-derived amino acid sequence is about 32000 Da | Mycolicibacterium smegmatis |
37000 | - |
SDS-PAGE | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephadex G-100 gel filtration | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.74 | - |
crude extract, at 50°C | Mycolicibacterium smegmatis |
16.2 | - |
after 22fold purification, at 50°C | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Met-p-nitroanilide + H2O | - |
Mycolicibacterium smegmatis | L-Met + p-nitroaniline | - |
? | |
L-Met-p-nitroanilide + H2O | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | L-Met + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MetAP | - |
Mycolicibacterium smegmatis |
methionine aminopeptidase | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
optimum temperature for MetAP activity against the substrate L-Met p-nitroanilide | Mycolicibacterium smegmatis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 65 | the enzyme is stable at 50 °C with half-life of more than 8 h, at 50°C and 55°C, the enzyme shows stable activity over a period of 1 h with only a slight decrease of 10% in activity, at 60 °C the relative activity decreases to 50% within 50 min, whereas at 65°C the relative activity decreases to 19% and declines to 0 after a period of 50 min | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mycolicibacterium smegmatis |