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Literature summary for 3.4.11.18 extracted from

  • Frottin, F.; Martinez, A.; Peynot, P.; Mitra, S.; Holz, R.C.; Giglione, C.; Meinnel, T.
    The proteomics of N-terminal methionine cleavage (2006), Mol. Cell. Proteomics, 5, 2336-2349.
    View publication on PubMed
Search for more literature for 3.4.11.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli from plasmid pXL1071 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 Met-Ala-Met-Lys-Ser at 30°C Escherichia coli
0.9
-
 Met-Gly-Met-Met at 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Superose-6 column chromatography, and Q-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Ala-Met-Lys-Ser + H2O
-
 Escherichia coli Met + Ala-Met-Lys-Ser
-
 ?
Met-Gly-Met-Met + H2O
-
 Escherichia coli Met + Gly-Met-Met
-
 ?
additional information peptides with Val or Thr at P1' are much less efficiently cleaved than those with Ala, Cys, Gly, Pro, or Ser in this position Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
methionine aminopeptidase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
 Met-Ala-Met-Lys-Ser at 30°C Escherichia coli
38
-
 Met-Gly-Met-Met at 30°C Escherichia coli