Protein Variants | Comment | Organism |
---|---|---|
H178A | the mutant enzyme binds only one equivalent of Co(II) or Fe(II) tightly with affinities that are identical to the wild-type. The turnover-number for Co(II) loaded H178A decreases 70fold towards Met-Gly-Met-Met ans 290fold towards Met-Pro-p-nitroanilide. The KM-value for Met-Gly-Met-Met remains unaffected, while that for Met-Pro-p-nitroanilide increases approximately 2fold for Co(II) - and Fe(II)-loaded H178A. The ratio of turnover-number to Km-value for both Co(II)- and Fe(II)-loaded H178A towards both substrates ranges from 50fold to 580fold reduction | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
Met-Pro-4-nitroanilide | wild-type enzyme, Co(II) loaded, pH 7.5 | Escherichia coli | |
0.09 | - |
Met-Pro-4-nitroanilide | wild-type enzyme, Fe(II) loaded, pH 7.5 | Escherichia coli | |
0.12 | - |
Met-Pro-4-nitroanilide | mutant enzyme H178A, Co(II) loaded, pH 7.5 | Escherichia coli | |
0.13 | - |
Met-Pro-4-nitroanilide | mutant enzyme H178A, Fe(II) loaded, pH 7.5 | Escherichia coli | |
2 | - |
Met-Gly-Met-Met | wild-type enzyme and mutant enzyme H178A, Fe(II) loaded, pH 7.5 | Escherichia coli | |
3.2 | - |
Met-Gly-Met-Met | wild-type enzyme and mutant enzyme H178A, Co(II) loaded, pH 7.5 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Gly-Met-Met + H2O | - |
Escherichia coli | Gly-Met-Met + Met | - |
? | |
Met-Pro-4-nitroanilide + H2O | - |
Escherichia coli | Met + Pro-4-nitroanilide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Met-Pro-p-nitroanilide | mutant enzyme H178A, Co(II) loaded, pH 7.5 | Escherichia coli | |
0.01 | - |
Met-Pro-p-nitroanilide | mutant enzyme H178A, Fe(II) loaded, pH 7.5 | Escherichia coli | |
0.54 | - |
Met-Gly-Met-Met | mutant enzyme H178A, Co(II) loaded, pH 7.5 | Escherichia coli | |
0.56 | - |
Met-Gly-Met-Met | mutant enzyme H178A, Fe(II) loaded, pH 7.5 | Escherichia coli | |
0.59 | - |
Met-Pro-p-nitroanilide | wild-type enzyme, Co(II) loaded, pH 7.5 | Escherichia coli | |
1.4 | - |
Met-Pro-p-nitroanilide | wild-type enzyme, Fe(II) loaded, pH 7.5 | Escherichia coli | |
28 | - |
Met-Gly-Met-Met | wild-type enzyme, Fe(II) loaded, pH 7.5 | Escherichia coli | |
39 | - |
Met-Gly-Met-Met | wild-type enzyme, Co(II) loaded, pH 7.5 | Escherichia coli |