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Literature summary for 3.4.11.18 extracted from

  • Chiu, C.H.; Lee, C.Z.; Lin, K.S.; Tam, M.F.; Lin, L.Y.
    Amino acid residues involved in the functional integrity of Escherichia coli methionine aminopeptidase (1999), J. Bacteriol., 181, 4686-4689.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.18

Protein Variants

Protein Variants Comment Organism
C59S 108% of the activity compared to wild-type enzyme Escherichia coli
C70S 54% of the activity compared to wild-type enzyme Escherichia coli
D108A 2% of the activity compared to the wild-type enzyme, cobalt content is 6% of that of the wild-type enzyme Escherichia coli
D97A 4% of the activity compared to the wild-type enzyme, cobalt content is not detectable Escherichia coli
E204V 5% of the activity compared to the wild-type enzyme, cobalt content is not detectable Escherichia coli
E235V 3% of the activity compared to the wild-type enzyme, cobalt content is 3% of that of the wild-type enzyme Escherichia coli
F177L 123% of the activity compared to wild-type enzyme Escherichia coli
H171L 6% of the activity compared to the wild-type enzyme, cobalt content is 6% of that of the wild-type enzyme Escherichia coli
W221L 27% of the activity compared to wild-type enzyme Escherichia coli
Y62F 110% of the activity compared to wild-type enzyme Escherichia coli
Y65F 98% of the activity compared to wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2144
-
 Met-Gly-Met-Met wild-type enzyme Escherichia coli
0.457
-
 Met-Gly-Met-Met mutant enzyme C70S Escherichia coli
0.6559
-
 Met-Gly-Met-Met mutant enzyme W221L Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ in mutant enzymes D97A and E204V cobalt content is not detectable, in mutant enzyme H171L and 235V the cobalt content is 3% of that of the wild-type enzyme, in mutant enzyme D108A the cobalt content is 6% of that of the wild-type enzyme Escherichia coli
Co2+ Co2+-dependent metallopeptidase Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Gly-Met-Met + H2O
-
 Escherichia coli Gly-Met-Met + Met
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.67
-
 Met-Gly-Met-Met mutant enzyme W221L Escherichia coli
0.78
-
 Met-Gly-Met-Met mutant enzyme C70S Escherichia coli
1.16
-
 Met-Gly-Met-Met wild-type enzyme Escherichia coli