Literature summary for 3.4.11.15 extracted from

Munih, P.; Moulin, A.; Stamper, C.C.; Bennett, B.; Ringe, D.; Petsko, G.A.; Holz, R.C.
X-ray crystallographic characterization of the Co(II)-substituted Tris-bound form of the aminopeptidase from Aeromonas proteolytica (2007), J. Inorg. Biochem., 101, 1099-1107.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
x-ray crystal structure of the Co(II)-loaded form ([CoCo(AAP)]) is solved to 2.2 A resolution. [CoCo(AAP)] folds into an alpha/beta globular domain with a twisted beta-sheet hydrophobic core sandwiched between alpha-helices, identical to [ZnZn(AAP)]. Tris(hydroxymethyl)aminomethane coordinates to the dinuclear Co(II) active site of AAP with one of the Tris hydroxyl oxygen atoms (O4) forming a single oxygen atom bridge between the two Co(II) ions. This is the only Tris atom coordinated to the metals with Co1-O and Co2-O bonds distances of 2.2 and 1.9 A, respectively. Each of the Co(II) ions resides in a distorted trigonal bipyramidal geometry	Vibrio proteolyticus

Crystallization (Comment)

Organism

x-ray crystal structure of the Co(II)-loaded form ([CoCo(AAP)]) is solved to 2.2 A resolution. [CoCo(AAP)] folds into an alpha/beta globular domain with a twisted beta-sheet hydrophobic core sandwiched between alpha-helices, identical to [ZnZn(AAP)]. Tris(hydroxymethyl)aminomethane coordinates to the dinuclear Co(II) active site of AAP with one of the Tris hydroxyl oxygen atoms (O4) forming a single oxygen atom bridge between the two Co(II) ions. This is the only Tris atom coordinated to the metals with Co1-O and Co2-O bonds distances of 2.2 and 1.9 A, respectively. Each of the Co(II) ions resides in a distorted trigonal bipyramidal geometry

Vibrio proteolyticus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	for spectroscopic studies the enzyme containing magnetically and spectroscopically silent Zn(II) ion is substituted with Co(II)	Vibrio proteolyticus
Zn2+	native protein	Vibrio proteolyticus

Organism

Organism	UniProt	Comment	Textmining
Vibrio proteolyticus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-leucine p-nitroanilide + H2O	-	Vibrio proteolyticus	L-leucine + p-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
AAP	-	Vibrio proteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Vibrio proteolyticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Vibrio proteolyticus