Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | above 3-7 mM | Hathewaya histolytica | |
CoSO4 | at high concentration, Co-citrate protects | Hathewaya histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | 3 | L-leucine 4-nitroanilide | - |
Hathewaya histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required, 0.93-1.25 mol cobalt per mol enzyme, optimal concentration 0.003-0.018 mM, involved in enzyme-substrate binding, inhibitory above 3-7 mM, time-course of activation | Hathewaya histolytica | |
Mn2+ | required | Hathewaya histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptides + H2O | Hathewaya histolytica | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hathewaya histolytica | - |
soluble and glass- or cellulose-immobilized enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of any N-terminal amino acid, including proline and hydroxyproline, but no cleavage of Xaa-Pro- | release of any N-terminal amino acid, including Pro and hydroxyproline, but no cleavage of XaaPro, mechanism involves Co2+ | Hathewaya histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Leu-4-nitroanilide + H2O | - |
Hathewaya histolytica | Leu + 4-nitroaniline | - |
ir | |
Peptides + H2O | - |
Hathewaya histolytica | ? | - |
? | |
Pro-Gly-Pro + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro | - |
ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
L-leucine 4-nitroanilide | - |
Hathewaya histolytica |