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Literature summary for 3.4.11.13 extracted from

  • Woessner, J.F.
    Clostridial aminopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2nd. Ed., 1011-1012.
No PubMed abstract available
Search for more literature for 3.4.11.13

General Stability

General Stability Organism
not stable to lyophilization Hathewaya histolytica

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibits in presence of Mg2+ Hathewaya histolytica
Lys(Abz)-Phe-p-nitrobenzyloxy
-
 Hathewaya histolytica
p-mercuribenzoate 1.7 mM, complete inhibition Hathewaya histolytica
Zn2+ inhibits in presence of Mg2+ Hathewaya histolytica

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates to a lesser extent than Mn2+, no activity in absence of metal ions Hathewaya histolytica
Mn2+ activates, no activity in absence of metal ions Hathewaya histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 6 * 56000, SDS-PAGE Hathewaya histolytica
340000
-
 gel filtration Hathewaya histolytica

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hathewaya histolytica

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Hathewaya histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Gly-Pro)n + H2O
-
 Hathewaya histolytica Pro + Gly-Pro-(Pro-Gly-Pro)n-1
-
 ?
(Pro-Gly-Pro)n-methyl ester + H2O
-
 Hathewaya histolytica Pro + Gly-Pro-(Pro-Gly-Pro)n-methyl ester
-
 ?
additional information enzyme can not remove the terminal Pro from poly-L-Pro. The enzyme is strictly an aminopeptidase and has no endopeptidase action on polypeptides and proteins. The amino group of the N-terminal amino acid must be free. Di-, tri- and polypeptide substrates are cleaved and the optimum size accomodated by the active center is four residues. The residues cleaved most rapidly are hydrophobic ones Hathewaya histolytica ?
-
 ?
Pro-Gly-Pro + H2O
-
 Hathewaya histolytica Pro + Gly-Pro
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 56000, SDS-PAGE Hathewaya histolytica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 Lys(Abz)-Phe-p-nitrobenzyloxy
-
 Hathewaya histolytica