Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-Cyclohexanedione | Ki: 0.022-0.023 mM | Vibrio proteolyticus | |
2,3-Butanedione | photochemical inactivation: effect only in the light, proportional to light intensity, modification of 5 Trp-, 3-4 Tyr-, 2 His- and 2Arg-residues, no photoinactivation in the absence of oxygen or in the presence of azide, protection also by Trp, Met, D-Met, L-2-thiol-His, 2-mercaptoethanol, Gly-Met, Ki: 5.1-19.8 mM | Vibrio proteolyticus | |
2,3-Pentanedione | photochemical inactivation: effect only in UV-light | Vibrio proteolyticus | |
3-methyl-1,2-cyclopentanedione | Ki: 0.48-1.19 mM | Vibrio proteolyticus | |
Glyoxal | Ki: 3.7-6.5 mM | Vibrio proteolyticus | |
methylglyoxal | photochemical inactivation: effect only in UV-light, Ki: 1.8-2.0 mM | Vibrio proteolyticus | |
Phenylglyoxal | Ki: 0.002-0.0023 mM | Vibrio proteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
L-leucine 4-nitroanilide | native enzyme | Vibrio proteolyticus | |
0.126 | - |
L-leucine 4-nitroanilide | photoinactivated enzyme | Vibrio proteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | no loss of zinc upon photoinactivation | Vibrio proteolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptides + H2O | Vibrio proteolyticus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptides + H2O | - |
Vibrio proteolyticus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
L-leucine 4-nitroanilide | photoinactivated enzyme | Vibrio proteolyticus | |
67.3 | - |
L-leucine 4-nitroanilide | native enzyme | Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Vibrio proteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | 0.0023 | Phenylglyoxal | - |
Vibrio proteolyticus | |
0.022 | 0.023 | 1,2-Cyclohexanedione | - |
Vibrio proteolyticus | |
0.48 | 1.19 | 3-methyl-1,2-cyclopentanedione | - |
Vibrio proteolyticus | |
1.8 | 2 | methylglyoxal | photochemical inactivation: effect only in UV-light | Vibrio proteolyticus | |
3.7 | 6.5 | Glyoxal | - |
Vibrio proteolyticus | |
5.1 | 19.8 | 2,3-Butanedione | photochemical inactivation: effect only in the light, proportional to light intensity, modification of 5 Trp-, 3-4 Tyr-, 2 His- and 2Arg-residues, no photoinactivation in the absence of oxygen or in the presence of azide, protection also by Trp, Met, D-Met, L-2-thiol-His, 2-mercaptoethanol, Gly-Met | Vibrio proteolyticus |