Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenylglyoxal | - |
Vibrio proteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | 0.0215 | L-leucine 4-nitroanilide | native enzyme, buffer dependent | Vibrio proteolyticus | |
0.032 | - |
L-leucine 4-nitroanilide | diethylpyrocarbonate-treated enzyme | Vibrio proteolyticus | |
0.055 | - |
L-leucine 4-nitroanilide | 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treated enzyme | Vibrio proteolyticus | |
0.06 | - |
L-leucine 4-nitroanilide | nitro-enzyme | Vibrio proteolyticus | |
0.136 | - |
L-leucine 4-nitroanilide | azo-enzyme | Vibrio proteolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptides + H2O | Vibrio proteolyticus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | - |
chemical modifications: nitration, azotization, diethylpyrocarbonate treatment, 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treatment | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. | presence of at least one active Tyr and one carboxyl group demonstrated by chemical modifications of enzyme: nitration, azotization, diethyldirocarbonate treatment, 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treatment | Vibrio proteolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
azo-enzyme | Vibrio proteolyticus |
17 | - |
nitro-enzyme | Vibrio proteolyticus |
20 | - |
1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treated enzyme | Vibrio proteolyticus |
80 | 115 | native enzyme, buffer dependent | Vibrio proteolyticus |
84 | - |
diethylpyrocarbonate-treated enzyme | Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Peptides + H2O | - |
Vibrio proteolyticus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
L-leucine 4-nitroanilide | azo-enzyme | Vibrio proteolyticus | |
14.3 | - |
L-leucine 4-nitroanilide | nitro-enzyme | Vibrio proteolyticus | |
42 | - |
L-leucine 4-nitroanilide | 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treated enzyme | Vibrio proteolyticus | |
50 | 69 | L-leucine 4-nitroanilide | native enzyme, buffer dependent | Vibrio proteolyticus | |
55.5 | - |
L-leucine 4-nitroanilide | diethylpyrocarbonate-treated enzyme | Vibrio proteolyticus |