Crystallization (Comment) | Organism |
---|---|
16 mg/ml enzyme complexed with inhibitory Tris, in 10 mM Tris, pH 8.0, 10 mM KSCN, 0.4 NaCl, vapour diffusion method, with precipitant solution containing 100 mM Tris, pH 8.0, 100 mM KSCN, 4.5 M NaCl, 48 h, X-ray diffraction structure determination and analysis at 1.2 A resolution | Vibrio proteolyticus |
purified enzyme in complex Tris, 16 mg/ml in 10 mM Tris, pH 8.0, 10 mM KSCN, and 0.4 M NaCl, hanging drop vapour diffusion method, precipitation solution contains 100 mM Tris, pH 8.0, 100 mM KSCN, and 4.5 M NaCl, 48 h, X-ray diffraction structure determination and analysis at 1.2 A resolution, modeling | Vibrio proteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tris | buffer inhibition; chelated to active site Zn2+ | Vibrio proteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | bound in the active site, binding structure, bridged bimetallic enzyme | Vibrio proteolyticus | |
Zn2+ | the enzyme is bridged bimetallic, the metal ions are involved in the reaction, overview | Vibrio proteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | Q01693 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. | active site structure, reaction mechanism | Vibrio proteolyticus | |
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. | active site structure, reaction mechanism involving the two zinc ions | Vibrio proteolyticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Vibrio proteolyticus | - |
Synonyms | Comment | Organism |
---|---|---|
AAP | - |
Vibrio proteolyticus |
Aeromonas proteolytica aminopeptidase | - |
Vibrio proteolyticus |
More | see also EC 3.4.11.22 | Vibrio proteolyticus |