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Literature summary for 3.4.11.1 extracted from

  • Gu, Y.Q.; Holzer, F.M.; Walling, L.
    Overexpression, purification and biochemical characterization of the wound-induced leucine aminopeptidase of tomato (1999), Eur. J. Biochem., 263, 726-735.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Solanum lycopersicum
amastatin
-
Solanum lycopersicum
bestatin
-
Solanum lycopersicum
EDTA
-
Solanum lycopersicum
Zn2+
-
Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
L-leucine-p-naphthylamide
-
Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
expressed in Escherichia coli Solanum lycopersicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
substrate leucine-p-naphthylamide Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Leu-2-naphthylamide + H2O
-
Solanum lycopersicum leucine + 2-naphthylamine
-
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