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Literature summary for 3.4.11.1 extracted from
- Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni (2018), Mol. Biochem. Parasitol., 219, 17-23 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | slight activation | Schistosoma mansoni |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lap1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Schistosoma mansoni |
| gene lap2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Schistosoma mansoni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | inhibits SmLAP2 | Schistosoma mansoni | |
| bestatin | a specific inhibitor of aminopeptidase; a specific inhibitor of aminopeptidase, complete inhibition | Schistosoma mansoni | |
| EDTA | inhibits SmLAP2, complete inhibition | Schistosoma mansoni | |
| PMSF | - |
Schistosoma mansoni | |
| trans-epoxy-succinyl-L-leucylamido(4-guanidino)butane | E64 | Schistosoma mansoni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Schistosoma mansoni | |
| Mn2+ | activates | Schistosoma mansoni | |
| Zn2+ | required | Schistosoma mansoni | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma mansoni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Schistosoma mansoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adult | - |
Schistosoma mansoni | - |
| egg | - |
Schistosoma mansoni | - |
| epithelium | - |
Schistosoma mansoni | - |
| gut | lumen | Schistosoma mansoni | - |
| intestine | - |
Schistosoma mansoni | - |
| intestine | presence of SmLAP1 in the lumen of the gut and in the subtegument | Schistosoma mansoni | - |
| miracidium | - |
Schistosoma mansoni | - |
| additional information | SmLAP1 and SmLAP2 expression in the egg and adult developmental stages of Schistosoma mansoni, from livers collected from mice 7 weeks post-infection, and in intestinal epithelia, vitelline cells and sub-tegumental regions of the parasite. Schistosoma mansoni LAPs show strong expression in the mammalian stages, i.e. schistosomules, male and female adults worms. Isozyme SmLAP2 shows higher expression than SmLAP1 in miracidia and sporocysts. Although both genes are expressed throughout the cycle, SmLAP2 is highly expressed in the molluscan infective free-living developmental stage (miracidium) and intrasnail stages. SmLAP1 is predominantly localized to gastrodermal cells lining the caecum, where digestion of hemoglobin from ingested host blood takes place | Schistosoma mansoni | - |
| sporocyst | - |
Schistosoma mansoni | - |
| subcutaneous tissue | - |
Schistosoma mansoni | - |
| vitelline cell | - |
Schistosoma mansoni | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Arg 7-amido-4-methylcoumarin + H2O | moderate activity | Schistosoma mansoni | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
| L-Leu 7-amido-4-methylcoumarin + H2O | best substrate | Schistosoma mansoni | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
| L-Leu 7-amido-4-methylcoumarin + H2O | fluorogenic substrate assay | Schistosoma mansoni | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
| L-Met 7-amido-4-methylcoumarin + H2O | moderate activity | Schistosoma mansoni | L-Met + 7-amino-4-methylcoumarin | - |
? | |
| L-Phe 7-amido-4-methylcoumarin + H2O | low activity | Schistosoma mansoni | L-Phe + 7-amino-4-methylcoumarin | - |
? | |
| L-Thr 7-amido-4-methylcoumarin + H2O | low activity | Schistosoma mansoni | L-Thr + 7-amino-4-methylcoumarin | - |
? | |
| L-Tyr 7-amido-4-methylcoumarin + H2O | lower activity | Schistosoma mansoni | L-Tyr + 7-amino-4-methylcoumarin | - |
? | |
| additional information | substrate specificity of SmLAP2, fluorogenic substrate assay, overview. No or poor activity with L-Pro 7-amido-4-methylcoumarin, L-Gly 7-amido-4-methylcoumarin, and L-Ser 7-amido-4-methylcoumarin | Schistosoma mansoni | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56700, about, sequence calculation, x * 60000, recombinant His-tagged SmLAP1, SDS-PAGE | Schistosoma mansoni |
| ? | x * 59700, about, sequence calculation, x * 63000, recombinant His-tagged SmLAP2, SDS-PAGE | Schistosoma mansoni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SmLAP1 | - |
Schistosoma mansoni |
| SmLAP2 | - |
Schistosoma mansoni |
| Smp_03000 | - |
Schistosoma mansoni |
| Smp_083870 | - |
Schistosoma mansoni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Schistosoma mansoni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Schistosoma mansoni |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Schistosoma mansoni | sequence calculation | - |
6.3 |
| Schistosoma mansoni | sequence calculation | - |
7.58 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the leucine aminopeptidases (LAPs) are members of the M17 family of Zn metalloproteases | Schistosoma mansoni |
| physiological function | isozyme SmLAP1 is involved in digestion | Schistosoma mansoni |
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