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Literature summary for 3.4.11.1 extracted from

  • Ratnayake, S.; Selvarkumar, P.; Hayashi, K.
    A putative proline iminopeptidase of Thermotoga maritima is a leucine aminopeptidase with lysine-p-nitroanilide hydrolyzing activity (2003), Enzyme Microb. Technol., 32, 414-421 .
No PubMed abstract available
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 0.1 mM, 93% loss of activity Thermotoga maritima
Co2+ 0.1 mM, 86% loss of activity Thermotoga maritima
Cu2+ 0.1 mM, 86% loss of activity Thermotoga maritima
EDTA 0.1 mM, complete inhibition Thermotoga maritima
Pb2+ 0.1 mM, 84% loss of activity Thermotoga maritima
Zn2+ 0.1 mM, 93% loss of activity Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
 L-Lys-4-nitroanilide pH 7.5, 35°C Thermotoga maritima
6.3
-
 L-Leu-4-nitroanilide pH 7.5, 35°C Thermotoga maritima
8.9
-
 L-Lys-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
12.7
-
 L-Leu-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
13.1
-
 L-Pro-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
24.7
-
 L-Ala-4-nitroanilide pH 7.5, 80°C Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ magnesium containing metallopeptidase Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WXP9
-
-
Thermotoga maritima DSM 3109 Q9WXP9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.5
-
 pH 7.5, 80°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O
-
 Thermotoga maritima L-alanine + 4-nitroaniline
-
 ?
L-Ala-4-nitroanilide + H2O
-
 Thermotoga maritima DSM 3109 L-alanine + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Thermotoga maritima L-leucine + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Thermotoga maritima DSM 3109 L-leucine + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide Thermotoga maritima L-lysine + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide Thermotoga maritima DSM 3109 L-lysine + 4-nitroaniline
-
 ?
L-Pro-4-nitroanilide + H2O very low activity Thermotoga maritima L-proline + 4-nitroaniline
-
 ?
L-Pro-4-nitroanilide + H2O very low activity Thermotoga maritima DSM 3109 L-proline + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
leucine aminopeptidase
-
 Thermotoga maritima
TM0042
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 thermostable up to 90°C at pH 7.5 for 30 min Thermotoga maritima
95
-
 20 min, 20% loss of activity Thermotoga maritima
100
-
 20 min, 58% loss of activity Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0104
-
 L-Lys-4-nitroanilide pH 7.5, 35°C Thermotoga maritima
0.0157
-
 L-Leu-4-nitroanilide pH 7.5, 35°C Thermotoga maritima
0.021
-
 L-Pro-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
0.078
-
 L-Ala-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
0.194
-
 L-Lys-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
0.285
-
 L-Leu-4-nitroanilide pH 7.5, 80°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8 30 min, stable between pH 6.5 and 8 at 80°C Thermotoga maritima
7.5
-
 optimal stability Thermotoga maritima
10
-
 30 min, 70% loss of activity Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0016
-
 L-Pro-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
0.0021
-
 L-Lys-4-nitroanilide pH 7.5, 35°C Thermotoga maritima
0.0025
-
 L-Leu-4-nitroanilide pH 7.5, 35°C Thermotoga maritima
0.0032
-
 L-Ala-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
0.0218
-
 L-Lys-4-nitroanilide pH 7.5, 80°C Thermotoga maritima
0.024
-
 L-Leu-4-nitroanilide pH 7.5, 80°C Thermotoga maritima