Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Clonorchis sinensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | - |
Clonorchis sinensis | |
bestatin | - |
Clonorchis sinensis | |
EDTA | - |
Clonorchis sinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
L-Leu-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.036 | - |
L-Leu-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.053 | - |
L-Met-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.0597 | - |
L-Met-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.0842 | - |
L-Phe-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.091 | - |
L-Phe-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.094 | - |
L-Lys-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.103 | - |
L-Lys-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.185 | - |
L-Ala-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.198 | - |
L-Thr-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.202 | - |
L-Ala-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.204 | - |
L-Thr-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.216 | - |
L-Pro-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.239 | - |
L-Pro-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.261 | - |
L-Ile-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.372 | - |
L-Val-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enhances activity | Clonorchis sinensis | |
Mn2+ | enhances activity | Clonorchis sinensis | |
Zn2+ | enhances activity | Clonorchis sinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clonorchis sinensis | C6FWG9 | - |
- |
Clonorchis sinensis | C6FWH0 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Clonorchis sinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelial cell | co-localizes with CsLAP1 to the intestinal epithelial cells of the parasite | Clonorchis sinensis | - |
epithelial cell | co-localizes with CsLAP2 to the intestinal epithelial cells of the parasite | Clonorchis sinensis | - |
gastrodermis | co-localizes with CsLAP1 to the gastrodermis of the parasite | Clonorchis sinensis | - |
gastrodermis | co-localizes with CsLAP2 to the gastrodermis of the parasite | Clonorchis sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Ala + 4-nitroaniline | - |
? | |
L-Ile-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Ile + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Leu + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Lys + 4-nitroaniline | - |
? | |
L-Met-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Met + 4-nitroaniline | - |
? | |
L-Phe-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Phe + 4-nitroaniline | - |
? | |
L-Pro-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Pro + 4-nitroaniline | - |
? | |
L-Thr-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Thr + 4-nitroaniline | - |
? | |
L-Val-4-nitroanilide + H2O | - |
Clonorchis sinensis | L-Val + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CsLAP1 | - |
Clonorchis sinensis |
CsLAP2 | - |
Clonorchis sinensis |
leucine aminopeptidase | - |
Clonorchis sinensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Clonorchis sinensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-Val-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.01 | - |
L-Ile-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.01 | - |
L-Thr-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.02 | - |
L-Pro-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.02 | - |
L-Thr-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.04 | - |
L-Phe-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.05 | - |
L-Lys-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.05 | - |
L-Phe-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.07 | - |
L-Lys-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.12 | - |
L-Ala-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.18 | - |
L-Met-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.2 | - |
L-Met-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.23 | - |
L-Leu-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.27 | - |
L-Leu-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Clonorchis sinensis |
Organism | Comment | Expression |
---|---|---|
Clonorchis sinensis | expression at transcription levels increases gradually in accordance with the maturation of the parasite | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
L-Val-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.038 | - |
L-Ile-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.049 | - |
L-Thr-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.083 | - |
L-Pro-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.092 | - |
L-Pro-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.1 | - |
L-Thr-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.44 | - |
L-Phe-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.53 | - |
L-Lys-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.59 | - |
L-Ala-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.59 | - |
L-Phe-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.6 | - |
L-Ala-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
0.68 | - |
L-Lys-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
3 | - |
L-Met-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
3.7 | - |
L-Met-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
6.3 | - |
L-Leu-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis | |
7.7 | - |
L-Leu-4-nitroanilide | pH 8, 20°C | Clonorchis sinensis |