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Literature summary for 3.4.11.1 extracted from

  • Georgiadou, D.; Hearn, A.; Evnouchidou, I.; Chroni, A.; Leondiadis, L.; York, I.A.; Rock, K.L.; Stratikos, E.
    Placental leucine aminopeptidase efficiently generates mature antigenic peptides in vitro but in patterns distinct from endoplasmic reticulum aminopeptidase 1 (2010), J. Immunol., 185, 1584-1592.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
a myc-His-tagged soluble version starting at amino acid position 155 (lacking the putative transmembrane region) expressed in 293F cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
233000 mol L-leucine-7-amido-4-methyl coumarin/mol enzyme x min Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine-7-amido-4-methylcoumarin + H2O
-
Homo sapiens L-leucine + 7-amino-4-methylcoumarin
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
-
Homo sapiens ? + Ile-Asn-Phe-Glu-Lys-Leu
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
-
Homo sapiens ? + Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O trimming of further model epitope precursors to antigenic peptides are also tested Homo sapiens L-leucine + Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
?

Synonyms

Synonyms Comment Organism
Placental leucine aminopeptidase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 almost 70% activity at pH 6.0, 50% activity at pH 8.0 Homo sapiens

General Information

General Information Comment Organism
physiological function enzymatic properties to participate in generating or destroying MHC class I-presented peptides Homo sapiens