Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of GST-tagged LAP in Escherichia coli DH12S cells | Babesia gibsoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | - |
Babesia gibsoni | |
Ca2+ | - |
Babesia gibsoni | |
Cu2+ | - |
Babesia gibsoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
L-proline-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.095 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.102 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.108 | - |
L-phenylalanine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.109 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, native enzyme | Babesia gibsoni | |
0.189 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates moderately | Babesia gibsoni | |
Mg2+ | activates slightly | Babesia gibsoni | |
Mn2+ | activates, best divalent cation | Babesia gibsoni | |
additional information | the enzyme is strongly dependent on metal divalent cations | Babesia gibsoni | |
Ni2+ | activates slightly | Babesia gibsoni | |
Zn2+ | activates slightly | Babesia gibsoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE | Babesia gibsoni |
85000 | - |
x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE | Babesia gibsoni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Babesia gibsoni | C5NSV0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
merozoite | - |
Babesia gibsoni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine-4-methylcoumaryl-7-amide + H2O | - |
Babesia gibsoni | L-alanine + 7-amino-4-methylcoumarine | - |
? | |
L-arginine-4-methylcoumaryl-7-amide + H2O | - |
Babesia gibsoni | L-arginine + 7-amino-4-methylcoumarine | - |
? | |
L-leucine-4-methylcoumaryl-7-amide + H2O | - |
Babesia gibsoni | L-leucine + 7-amino-4-methylcoumarine | - |
? | |
L-phenylalanine-4-methylcoumaryl-7-amide + H2O | - |
Babesia gibsoni | L-phenylalanine + 7-amino-4-methylcoumarine | - |
? | |
L-proline-4-methylcoumaryl-7-amide + H2O | - |
Babesia gibsoni | L-proline + 7-amino-4-methylcoumarine | - |
? | |
additional information | substrate specificity, overview | Babesia gibsoni | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE | Babesia gibsoni |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Babesia gibsoni |
leucine aminopeptidase | - |
Babesia gibsoni |
M17 leucyl aminopeptidase | - |
Babesia gibsoni |
More | the enzyme belongs to the M17 family leucine aminopeptidases | Babesia gibsoni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Babesia gibsoni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.03 | - |
L-proline-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
8.2 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
24.61 | - |
L-phenylalanine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
37.79 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
198.8 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Babesia gibsoni |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | activity range | Babesia gibsoni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
L-proline-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.086 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.227 | - |
L-phenylalanine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.752 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
1.942 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Babesia gibsoni |