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Literature summary for 3.4.11.1 extracted from

  • Jia, H.; Nishikawa, Y.; Luo, Y.; Yamagishi, J.; Sugimoto, C.; Xuan, X.
    Characterization of a leucine aminopeptidase from Toxoplasma gondii (2010), Mol. Biochem. Parasitol., 170, 1-6.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree, expression of GST-tagged LAP in Escherichia coli strain BL21, construction of a HA-tagged enzyme Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
 Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 L-phenylalanine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.107
-
 L-leucine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.124
-
 L-proline-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.169
-
 L-alanine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.426
-
 L-arginine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Toxoplasma gondii 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Toxoplasma gondii
Mn2+ activates, best metal ion Toxoplasma gondii
additional information the enzyme activity strongly depends on metal ions, only slight activition by Fe2+, Mg2+, Ca2+, Cu2+, and Zn2+ Toxoplasma gondii
Ni2+ activates Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE Toxoplasma gondii
85000
-
 x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE Toxoplasma gondii
500000
-
 recombinant enzyme, native PAGE Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-
Toxoplasma gondii RH
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged LAP from Escherichia coli strain BL21 by glutathione affinity chromatography Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-alanine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii RH L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-arginine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii L-arginine + 7-amino-4-methylcoumarin
-
 ?
L-arginine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii RH L-arginine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii RH L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii RH L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?
L-proline-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii L-proline + 7-amino-4-methylcoumarin
-
 ?
L-proline-4-methylcoumarin-7-amide + H2O
-
 Toxoplasma gondii RH L-proline + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE Toxoplasma gondii

Synonyms

Synonyms Comment Organism
LAP
-
 Toxoplasma gondii
leucine aminopeptidase
-
 Toxoplasma gondii
More the enzyme belongs to the M17 family leucine aminopeptidases Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0002
-
 L-phenylalanine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.0016
-
 L-proline-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.03
-
 L-alanine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.041
-
 L-arginine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.089
-
 L-leucine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Toxoplasma gondii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0023
-
 L-phenylalanine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.013
-
 L-proline-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.095
-
 L-arginine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.18
-
 L-alanine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii
0.832
-
 L-leucine-4-methylcoumarin-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Toxoplasma gondii