Literature summary for 3.4.11.1 extracted from

Song, S.M.; Park, J.H.; Kim, J.; Kim, S.I.; Hong, Y.C.; Kong, H.H.; Chung, D.I.
Identification and characterization of Paragonimus westermani leucine aminopeptidase (2008), Parasitol. Int., 57, 334-341.

Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Paragonimus westermani

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	30.4% residual activity at 1 mM	Paragonimus westermani
bestatin	18.9% residual activity at 0.05 mM	Paragonimus westermani
EDTA	21.3% residual activity at 10 mM	Paragonimus westermani
Zn2+	1-10 mM Zn2+ plus Mg2+ reduce the enzymatic activity	Paragonimus westermani

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	LAP activity is progressively enhanced by the addition of Mg2+ in the range of 0.001 mM to 1 mM	Paragonimus westermani
Mn2+	LAP activity is progressively enhanced by the addition of Mn2+ in the range of 0.001 mM to 1 mM	Paragonimus westermani
Zn2+	LAP enzyme activity is increased in the presence of 0.01 mM Zn2+	Paragonimus westermani

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	SDS-PAGE	Paragonimus westermani

Organism

Organism	UniProt	Comment	Textmining
Paragonimus westermani	A1Z0K2	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Paragonimus westermani

Source Tissue

Source Tissue	Comment	Organism	Textmining
eggshell	-	Paragonimus westermani	-
gut epithelium	-	Paragonimus westermani	-
subtegumental tissue	-	Paragonimus westermani	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Arg-7-amido-4-methylcoumarin + H2O	34.7% activity compared to L-Leu-7-amido-4-methylcoumarin	Paragonimus westermani	L-Arg + 7-amino-4-methylcoumarin	-	?
L-Leu-7-amido-4-methylcoumarin + H2O	100% activity, LAP protein demonstrates preferential substrate specificity for Leu-7-amido-4-methylcoumarin	Paragonimus westermani	L-Leu + 7-amino-4-methylcoumarin	-	?
L-Tyr-7-amido-4-methylcoumarin + H2O	6.4% activity compared to L-Leu-7-amido-4-methylcoumarin	Paragonimus westermani	L-Tyr + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
LAP	-	Paragonimus westermani
leucine aminopeptidase	-	Paragonimus westermani

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Paragonimus westermani

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Release 2023.1 (January 2023)