Crystallization (Comment) | Organism |
---|---|
Zn2+-Zn2+-enzyme in complex with the peptidomimetic derivative zofenoprilat, soaking of crystals in 20 mM zofenoprilat, X-ray diffraction structure determination and analysis at 1.85 A resolution | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cys-Gly | inhibits the activity of the Zn2+-Zn2+-enzyme with substrate Leu-Gly competitively | Bos taurus | |
Leu-Gly | inhibits the activity of the Mn2+-Zn2+-enzyme with substrate Cys-Gly competitively | Bos taurus | |
zofenoprilat | competitive versus Leu-Gly with the Zn2+-Zn2+-enzyme, uncompetitive with the Mn2+-Zn2+-enzyme, binding structure analysis | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | two ions per subunit, one exchangeable metal site can be occupied by Zn2+ or Mg2+, Co2+, or Mn2+, while the second, tight binding site can be occupied only by Zn2+ or Co2+, overview | Bos taurus | |
Mg2+ | two ions per subunit, one exchangeable metal site can be occupied by Zn2+ or Mg2+, Co2+, or Mn2+, while the second, tight binding site can be occupied only by Zn2+ or Co2+, overview | Bos taurus | |
Mn2+ | Km is 0.00056 mM with substrate Cys-Gly, two ions per subunit, one exchangeable metal site can be occupied by Zn2+ or Mg2+, Co2+, or Mn2+, while the second, tight binding site can be occupied only by Zn2+ or Co2+, overview | Bos taurus | |
additional information | metallopeptidase | Bos taurus | |
Zn2+ | two ions per subunit, one exchangeable metal site can be occupied by Zn2+ or Mg2+, Co2+, or Mn2+, while the second, tight binding site can be occupied only by Zn2+ or Co2+, overview | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00727 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of Zn2+-Zn2+-enzyme and Mn2+-Zn2+-enzyme, by ethanol precipitation, and gel filtration to homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lens | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme, in 10 mM sodium phosphate, pH 7.8, 0.02 M magnesium acetate, 2 mM DTT, and 0.05 mM manganese chloride, stable for at least 2 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cys-Gly + H2O | only hydrolyzed by the Mn2+-Zn2+-enzyme, not by the Zn2+-Zn2+-enzyme | Bos taurus | Cys + Gly | - |
? | |
Leu-Gly + H2O | substrate of the Mn2+-Zn2+-enzyme and the Zn2+-Zn2+-enzyme | Bos taurus | Leu + Gly | - |
? | |
additional information | the enzyme prefers peptide substrates with bulky and hydrophobic N-terminal amino acid residues | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
zofenoprilat | Zn2+-Zn2+-enzyme, versus substrate Leu-Gly | Bos taurus | |
0.0164 | - |
Cys-Gly | Mn2+-Zn2+-enzyme, versus substrate Leu-Gly | Bos taurus | |
0.65 | - |
Leu-Gly | Z2+-Zn2+-enzyme, versus substrate Cys-Gly | Bos taurus | |
1.1 | - |
zofenoprilat | Mn2+-Zn2+-enzyme, with substrate Leu-Gly | Bos taurus | |
1.3 | - |
zofenoprilat | Mn2+-Zn2+-enzyme, with substrate Cys-Gly | Bos taurus |