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Literature summary for 3.4.11.1 extracted from

  • Morty, R.E.; Morehead, J.
    Cloning and characterization of a leucyl aminopeptidase from three pathogenic Leishmania species (2002), J. Biol. Chem., 29, 26057-26065.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Leishmania donovani
constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Leishmania major
constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Leishmania amazonensis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strong inhibition Leishmania amazonensis
1,10-phenanthroline strong inhibition Leishmania donovani
1,10-phenanthroline strong inhibition Leishmania major
1,7-phenanthroline slight inhibition Leishmania amazonensis
1,7-phenanthroline slight inhibition Leishmania donovani
1,7-phenanthroline slight inhibition Leishmania major
actinonin tight-binding, competitive inhibitor Leishmania amazonensis
actinonin tight-binding, competitive inhibitor Leishmania donovani
actinonin tight-binding, competitive inhibitor Leishmania major
apstatin strong inhibition Leishmania amazonensis
apstatin strong inhibition Leishmania donovani
apstatin strong inhibition Leishmania major
arphamenine A non-tight-binding, competitive inhibitor Leishmania amazonensis
arphamenine A non-tight-binding, competitive inhibitor Leishmania donovani
arphamenine A non-tight-binding, competitive inhibitor Leishmania major
bestatin strong inhibition Leishmania amazonensis
bestatin strong inhibition Leishmania donovani
bestatin strong inhibition Leishmania major
Ca2+ complete inhibition at 0.1-10 mM Leishmania amazonensis
Cu2+ 93% inhibition at 1 mM Leishmania amazonensis
DL-thiorphan slight inhibition at 0.1 mM Leishmania amazonensis
DL-thiorphan slight inhibition at 0.1 mM Leishmania donovani
DL-thiorphan slight inhibition at 0.1 mM Leishmania major
EDTA strong inhibition Leishmania amazonensis
EDTA strong inhibition Leishmania donovani
EDTA strong inhibition Leishmania major
EGTA
-
Leishmania amazonensis
EGTA
-
Leishmania donovani
EGTA
-
Leishmania major
Fe2+ complete inhibition at 1-10 mM Leishmania amazonensis
GSH slight inhibition Leishmania donovani
GSH slight inhibition Leishmania major
L-Leucinol non-tight-binding, competitive inhibitor Leishmania amazonensis
L-Leucinol non-tight-binding, competitive inhibitor Leishmania donovani
L-Leucinol non-tight-binding, competitive inhibitor Leishmania major
additional information no inhibition by DTT and GSH, inhibition mechanisms Leishmania amazonensis
additional information no inhibition by DTT, inhibition mechanisms Leishmania donovani
additional information no inhibition by DTT, inhibition mechanisms Leishmania major
phosphoramidon slight inhibition at 0.1 mM Leishmania amazonensis
phosphoramidon slight inhibition at 0.1 mM Leishmania donovani
phosphoramidon slight inhibition at 0.1 mM Leishmania major
puromycin slight inhibition Leishmania amazonensis
puromycin slight inhibition Leishmania donovani
puromycin slight inhibition Leishmania major
Zn2+ inhibitory at 10 mM, slightly activating at 1 mM Leishmania amazonensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.044
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.054
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.06
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.062
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.072
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.077
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.104
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.105
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.118
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.137
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.154
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.167
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.189
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.229
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.392
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.408
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.442
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Leishmania amazonensis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates best at 1 mM Leishmania amazonensis
Mg2+ activates at 1-10 mM Leishmania amazonensis
Mn2+ activates at 0.1-1 mM Leishmania amazonensis
Ni2+ activates at 1-10 mM Leishmania amazonensis
Zn2+ bound to the enzyme as native cofactor Leishmania amazonensis
Zn2+ inhibitory at 10 mM, slightly activating at 1 mM Leishmania amazonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62337
-
6 * 62337, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania donovani
62424
-
6 * 62424, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania amazonensis
62569
-
6 * 62569, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania major
360000
-
gel filtration Leishmania amazonensis

Organism

Organism UniProt Comment Textmining
Leishmania amazonensis Q95V76
-
-
Leishmania donovani Q95V75
-
-
Leishmania major Q7KF27
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Leishmania donovani
additional information
-
-
Leishmania major
additional information
-
-
Leishmania amazonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-7-amido-4-methylcoumarin + H2O low activity Leishmania donovani L-Ala + 7-amino-4-methylcoumarin
-
?
L-Ala-7-amido-4-methylcoumarin + H2O low activity Leishmania major L-Ala + 7-amino-4-methylcoumarin
-
?
L-Ala-7-amido-4-methylcoumarin + H2O low activity Leishmania amazonensis L-Ala + 7-amino-4-methylcoumarin
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
-
Leishmania donovani L-Cys + 7-amino-4-methylcoumarin
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
-
Leishmania major L-Cys + 7-amino-4-methylcoumarin
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
-
Leishmania amazonensis L-Cys + 7-amino-4-methylcoumarin
-
?
L-Ile-7-amido-4-methylcoumarin + H2O low activity Leishmania donovani L-Ile + 7-amino-4-methylcoumarin
-
?
L-Ile-7-amido-4-methylcoumarin + H2O low activity Leishmania major L-Ile + 7-amino-4-methylcoumarin
-
?
L-Ile-7-amido-4-methylcoumarin + H2O low activity Leishmania amazonensis L-Ile + 7-amino-4-methylcoumarin
-
?
L-Leu-7-amido-4-methylcoumarin + H2O best synthetic substrate Leishmania donovani L-Leu + 7-amino-4-methylcoumarin
-
?
L-Leu-7-amido-4-methylcoumarin + H2O best synthetic substrate Leishmania major L-Leu + 7-amino-4-methylcoumarin
-
?
L-Leu-7-amido-4-methylcoumarin + H2O best synthetic substrate Leishmania amazonensis L-Leu + 7-amino-4-methylcoumarin
-
?
L-Met-7-amido-4-methylcoumarin + H2O
-
Leishmania donovani L-Met + 7-amino-4-methylcoumarin
-
?
L-Met-7-amido-4-methylcoumarin + H2O
-
Leishmania major L-Met + 7-amino-4-methylcoumarin
-
?
L-Met-7-amido-4-methylcoumarin + H2O
-
Leishmania amazonensis L-Met + 7-amino-4-methylcoumarin
-
?
L-Trp-7-amido-4-methylcoumarin + H2O low activity Leishmania donovani L-Trp + 7-amino-4-methylcoumarin
-
?
L-Trp-7-amido-4-methylcoumarin + H2O low activity Leishmania major L-Trp + 7-amino-4-methylcoumarin
-
?
L-Trp-7-amido-4-methylcoumarin + H2O low activity Leishmania amazonensis L-Trp + 7-amino-4-methylcoumarin
-
?
additional information no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide Leishmania donovani ?
-
?
additional information no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide Leishmania major ?
-
?
additional information no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide Leishmania amazonensis ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 62337, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania donovani
hexamer 6 * 62424, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania amazonensis
hexamer 6 * 62569, recombinant His-tagged enzyme, amino acid sequence calculation Leishmania major

Synonyms

Synonyms Comment Organism
LAP
-
Leishmania donovani
LAP
-
Leishmania major
LAP
-
Leishmania amazonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Leishmania donovani
37
-
assay at Leishmania major
37
-
assay at Leishmania amazonensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.057 0.65 L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.07
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.09
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.19
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.19
-
L-Ile-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.21
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
0.22
-
L-Trp-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
0.41
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
0.52
-
L-Leu-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
1.61
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
1.89
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
2.01
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
2.19
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
2.72
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
2.94
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
2.94
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
2.94
-
L-Ala-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
2.94
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major
3 6 L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3 6 L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.3
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
3.3
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
3.34
-
L-Cys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
4.99
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania amazonensis
5.08
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania donovani
5.52
-
L-Met-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant His-tagged enzyme Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Leishmania donovani
8
-
assay at Leishmania major
8.5
-
-
Leishmania amazonensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000008
-
actinonin
-
Leishmania donovani
0.000001
-
actinonin
-
Leishmania amazonensis
0.0000029
-
actinonin
-
Leishmania major
0.000003
-
bestatin
-
Leishmania amazonensis
0.000044
-
apstatin
-
Leishmania amazonensis
0.059
-
arphamenine A
-
Leishmania donovani
0.069
-
arphamenine A
-
Leishmania major
0.07
-
arphamenine A
-
Leishmania amazonensis
0.102
-
L-Leucinol
-
Leishmania major
0.109
-
L-Leucinol
-
Leishmania amazonensis
0.118
-
L-Leucinol
-
Leishmania donovani