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Literature summary for 3.4.11.1 extracted from
- Characterization of recombinant human adipocyte-derived leucine aminopeptidase expressed in chinese hamster ovary cells (2000), J. Biochem., 128, 755-762.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in COS-7 and in CHO cells, secretion from recombinant COS-7 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amastatin | - |
Homo sapiens |  |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.28 | - |
Leu-4-nitroanilide | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | zinc-metallopeptidase, contains the HEXXH motif | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 106000 | - |
1 * 106000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 120000 | - |
recombinant enzyme from CHO cells, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin II + H2O | Homo sapiens | - |
angiotensin IV + ? | through angiotensin III | ? | |
| kallidin + H2O | Homo sapiens | i.e. LRPPGFSPFR | Leu + bradykinin | i.e. RPPGFSPFR | ? | |
| additional information | Homo sapiens | broad substrate specificity towards naturally occurring peptide hormones, while the enzyme hydrolyzes synthetic substrate in vitro with specificity for leucine, enzyme plays a role in regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from CHO cells, 5fold to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Homo sapiens | - |
| kidney | cortex | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.192 | - |
purified recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin II + H2O | - |
Homo sapiens | angiotensin IV + ? | through angiotensin III | ? | |
| dynorphin A + H2O | - |
Homo sapiens | ? | - |
? | |
| kallidin + H2O | i.e. LRPPGFSPFR | Homo sapiens | Leu + bradykinin | i.e. RPPGFSPFR | ? | |
| Leu-4-nitroanilide + H2O | - |
Homo sapiens | Leu + 4-nitroaniline | - |
? | |
| additional information | broad substrate specificity towards naturally occurring peptide hormones, while the enzyme hydrolyzes synthetic substrate in vitro with specificity for leucine, enzyme plays a role in regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney | Homo sapiens | ? | - |
? | |
| Neurokinin A + H2O | - |
Homo sapiens | ? | - |
? | |
| Neuromedin B + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 106000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| A-LAP | - |
Homo sapiens |
| adipocyte-derived leucine aminopeptidase | - |
Homo sapiens |
| aminopeptidase I | - |
Homo sapiens |
| aminopeptidase II | - |
Homo sapiens |
| Aminopeptidase III | - |
Homo sapiens |
| cathepsin III | - |
Homo sapiens |
| cytosol aminopeptidase | - |
Homo sapiens |
| FTBL protein | - |
Homo sapiens |
| L-leucine aminopeptidase | - |
Homo sapiens |
| leucinamide aminopeptidase | - |
Homo sapiens |
| leucinaminopeptidase | - |
Homo sapiens |
| leucine aminopeptidase | - |
Homo sapiens |
| leucyl peptidase | - |
Homo sapiens |
| More | enzyme belongs to the peptidase family M17, enzyme belongs to the metallopeptidase family M1 | Homo sapiens |
| peptidase S | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profile | Homo sapiens |
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