General Stability | Organism |
---|---|
Mn2+ is absolutely required for stability during purification | Bos taurus |
Mn2+ is essential for enzyme stability, in absence of Mn2+ the enzyme is inactivated within 48 h | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
Cys-Gly | pH 6.9, 25°C | Bos taurus | |
0.57 | - |
Cys-Gly | pH 7.4, 25°C | Bos taurus | |
0.59 | - |
Cys-Gly | pH 8.3, 25°C | Bos taurus | |
1.3 | - |
L-Met-Gly | pH 8.3, 25°C | Bos taurus | |
1.5 | - |
Met-Gly | pH 7.4, 25°C | Bos taurus | |
1.5 | - |
Leu-Gly | pH 8.3, 25°C | Bos taurus | |
2.3 | - |
Leu-Gly | pH 6.9, 25°C | Bos taurus | |
2.5 | - |
Leu-Gly | pH 7.4, 25°C | Bos taurus | |
2.8 | - |
L-Met-Gly | pH 6.9, 25°C | Bos taurus | |
5 | - |
Ser-Gly | pH 8.3, 25°C | Bos taurus | |
5.2 | - |
L-Ser-Gly | pH 7.4, 25°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | absolutely dependent on | Bos taurus | |
Mn2+ | dependent on | Bos taurus | |
additional information | unaffected by Zn2+ after removal of bound metal ions, while Mn2+ can restore activity | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52874 | - |
6 * 53700, SDS-PAGE, 6 * 52874, mass spectrometry | Bos taurus |
52874 | - |
x * 52874, mass spectrometry | Bos taurus |
53700 | - |
6 * 53700, SDS-PAGE, 6 * 52874, mass spectrometry | Bos taurus |
320000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cys-Gly + H2O | Bos taurus | the enzyme plays a role in glutathione turnover | Cys + Gly | - |
? | |
cysteinylglycine + H2O | Bos taurus | involved in turnover of glutathione | cysteine + glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00727 | - |
- |
Purification (Comment) | Organism |
---|---|
1090fold, to homogeneity | Bos taurus |
native enzyme 1090fold from lens to homogeneity by ion exchange chromatography, ethanol and alkaline precipitation, chloroform/octanol extraction, and gel filtration | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low | molecular modelling of enzyme-substrate binding | Bos taurus | |
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low | substrate-enzyme complex structure, molecular modeling | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lens | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.9 | - |
purified enzyme | Bos taurus |
Storage Stability | Organism |
---|---|
-80°C, pure enzyme, 0.2 mM MnCl2, 2 weeks, stable | Bos taurus |
-80°C, purified enzyme after chloroform/octanol extraction purification step, in presence of 0.2 mM Mn2+, stable for at least 2 weeks | Bos taurus |
4°C, partially purified enzyme after ion exchange chromatography purification step, stable for at least 6 months | Bos taurus |
4°C, partially purified enzyme, 6 months, stable, less stable after further purification | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cys-Gly + H2O | - |
Bos taurus | Cys + Gly | - |
? | |
Cys-Gly + H2O | the enzyme plays a role in glutathione turnover | Bos taurus | Cys + Gly | - |
? | |
cysteinylglycine + H2O | involved in turnover of glutathione | Bos taurus | cysteine + glycine | - |
? | |
L-Met-Gly + H2O | - |
Bos taurus | L-Met + Gly | - |
? | |
L-Ser-Gly + H2O | - |
Bos taurus | L-Ser + Gly | - |
? | |
Leu-Gly + H2O | - |
Bos taurus | Leu + Gly | - |
? | |
Met-Gly + H2O | - |
Bos taurus | Met + Gly | - |
? | |
additional information | molecular modelling of enzyme-substrate conformation, no activity with Asp-Gly | Bos taurus | ? | - |
? | |
additional information | no activity with Ser-Gly and Asp-Gly | Bos taurus | ? | - |
? | |
Ser-Gly + H2O | - |
Bos taurus | Ser + Gly | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52874, mass spectrometry | Bos taurus |
hexamer | 6 * 53700, SDS-PAGE, 6 * 52874, mass spectrometry | Bos taurus |
More | primary structure analysis and molecular modeling | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
cysteinyl-glycine hydrolysing activity | - |
Bos taurus |
LAP | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
Cys-Gly | pH 6.9, 25°C | Bos taurus | |
17 | - |
Ser-Gly | pH 7.4, 25°C | Bos taurus | |
42 | - |
Ser-Gly | pH 8.3, 25°C | Bos taurus | |
56.66 | - |
Cys-Gly | pH 6.9, 25°C | Bos taurus | |
57 | - |
Cys-Gly | pH 6.9, 25°C | Bos taurus | |
67 | - |
Cys-Gly | pH 8.3, 25°C | Bos taurus | |
100 | - |
Cys-Gly | pH 7.4, 25°C | Bos taurus | |
118 | - |
Cys-Gly | pH 8.3, 25°C | Bos taurus | |
118.3 | - |
Cys-Gly | pH 8.3, 25°C | Bos taurus | |
135.8 | - |
Met-Gly | pH 6.9, 25°C | Bos taurus | |
136 | - |
Met-Gly | pH 6.9, 25°C | Bos taurus | |
191.7 | - |
Leu-Gly | pH 6.9, 25°C | Bos taurus | |
192 | - |
Leu-Gly | pH 6.9, 25°C | Bos taurus | |
400 | - |
Leu-Gly | pH 7.4, 25°C | Bos taurus | |
468 | - |
Met-Gly | pH 7.4, 25°C | Bos taurus | |
486.3 | - |
Met-Gly | pH 7.4, 25°C | Bos taurus | |
675 | - |
Leu-Gly | pH 8.3, 25°C | Bos taurus | |
988 | - |
Met-Gly | pH 8.3, 25°C | Bos taurus | |
988.3 | - |
Met-Gly | pH 8.3, 25°C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
modeling of pH-dependency | Bos taurus |
6.9 | 8.3 | assay at | Bos taurus |
7.4 | - |
assay at | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of activity with different dipeptide substrates | Bos taurus |