Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.1 extracted from

  • Delange, R.J.; Smith, E.L.
    Leucine aminopeptidase and other N-terminal exopeptidases (1971), The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 3, 81-118.
No PubMed abstract available
Search for more literature for 3.4.11.1

Inhibitors

Inhibitors Comment Organism Structure
1-butanol
-
 Bos taurus
1-butanol
-
 Sus scrofa
aliphatic alcohols methanol to n-hexanol Bos taurus
aliphatic alcohols methanol to n-hexanol Sus scrofa
alpha-keto-isocaproamide
-
 Bos taurus
alpha-keto-isocaproamide
-
 Sus scrofa
Cu2+
-
 Bos taurus
Cu2+
-
 Sus scrofa
D,L-Alpha-hydroxyisocaproamide
-
 Bos taurus
D,L-Alpha-hydroxyisocaproamide
-
 Sus scrofa
glycerol
-
 Bos taurus
glycerol
-
 Sus scrofa
Hg2+
-
 Bos taurus
Hg2+
-
 Sus scrofa
Isocaproic acid
-
 Bos taurus
Isocaproic acid
-
 Sus scrofa
L-leucine competitive Bos taurus
L-leucine competitive Sus scrofa
L-Leucinol
-
 Bos taurus
L-Leucinol
-
 Sus scrofa
Pb2+
-
 Bos taurus
Pb2+
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 L-Leu-L-Val
-
 Sus scrofa
1.56
-
 L-Leu-benzyl ester
-
 Sus scrofa
4.02
-
 leucinamide similar value Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Sus scrofa
Mg2+ activation Bos taurus
Mn2+ activation Sus scrofa
Mn2+ activation Bos taurus
Zn2+
-
 Sus scrofa
Zn2+
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Sus scrofa
-
 hog
-

Purification (Commentary)

Purification (Comment) Organism
overview procedures Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low mechanism Sus scrofa
a
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low mechanism Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
lens
-
 Bos taurus
-
pancreas
-
 Sus scrofa
-

Storage Stability

Storage Stability Organism
frozen, pH 7-9, presence of MgCl2, indefinitely Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-benzyl ester + H2O
-
 Sus scrofa L-Leu + benzylalcohol
-
 ?
L-leucine amide + H2O
-
 Sus scrofa L-leucine + NH3
-
 ?
Leu-Val + H2O
-
 Sus scrofa Leu + Val
-
 ?
additional information use in sequence determination Sus scrofa ?
-
 ?
additional information use in sequence determination Bos taurus ?
-
 ?
additional information no hydrolysis of Xaa-Pro bond Sus scrofa ?
-
 ?
additional information no hydrolysis of Xaa-Pro bond Bos taurus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.4
-
 Sus scrofa
9 9.4
-
 Bos taurus