Protein Variants | Comment | Organism |
---|---|---|
additional information | comparison of preparative resolution of (+)- and (-)-limonene oxide mixtures catalyzed by different LEHs under non-optimized conditions, optimization of the biocatalyzed processes to obtain enantiomerically pure isomers, overview | Rhodococcus erythropolis |
additional information | comparison of preparative resolution of (+)- and (-)-limonene oxide mixtures catalyzed by different LEHs under non-optimized conditions, optimization of the biocatalyzed processes, overview. The obtainment of the cis isomer from the same (+)-limonene oxide mixture by Tomsk-LEH-catalyzed resolution is not significantly improved | uncultured organism |
additional information | comparison of preparative resolution of (+)- and (-)-limonene oxide mixtures catalyzed by different LEHs under non-optimized conditions, optimization of the biocatalyzed processes, overview. The obtainment of the enantiomerically pure trans isomer, the increase of the reaction temperature to 50°C leads to an excellent resolution even at a substrate loading of 2 mol/l in reasonable reaction times | uncultured organism |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
limonene-1,2-epoxide + H2O | Rhodococcus erythropolis | - |
limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | uncultured organism | - |
limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | Rhodococcus erythropolis DCL14 | - |
limonene-1,2-diol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q9ZAG3 | - |
- |
Rhodococcus erythropolis DCL14 | Q9ZAG3 | - |
- |
uncultured organism | A0A0G3IAY2 | - |
- |
uncultured organism | A0A0G3ICV8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.097 | - |
Tomsk-LEH, substrate (+)-limonene oxide, pH 8.0, 20°C, non-optimized conditions | uncultured organism |
0.157 | - |
Tomsk-LEH, substrate (+)-limonene oxide, pH 8.0, 30°C, optimized conditions | uncultured organism |
0.23 | - |
CH55-LEH , substrate (-)-limonene oxide, pH 8.0, 20°C, non-optimized conditions | uncultured organism |
1.33 | - |
CH55-LEH , substrate (-)-limonene oxide, pH 8.0, 50°C, optimized conditions | uncultured organism |
1.85 | - |
Re-LEH , substrate (-)-limonene oxide, pH 8.0, 20°C, non-optimized conditions | Rhodococcus erythropolis |
3.17 | - |
Re-LEH , substrate (-)-limonene oxide, pH 8.0, 50°C, optimized conditions | Rhodococcus erythropolis |
6.18 | - |
Re-LEH , substrate (+)-limonene oxide, pH 8.0, 20°C, non-optimized conditions | Rhodococcus erythropolis |
13.95 | - |
Re-LEH , substrate (+)-limonene oxide, pH 8.0, 50°C, optimized conditions | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-limonene oxide + H2O | - |
uncultured organism | (1R,2R,4S)-limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | - |
Rhodococcus erythropolis | limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | - |
uncultured organism | limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | - |
Rhodococcus erythropolis DCL14 | limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | enantiomeric mixtures of (+)-limone oxide, overview. Enzyme CH55-LEH prefers the cis form of (-)-limonene oxide | uncultured organism | (1R,2R,4S)-limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | enantiomeric mixtures of (+)-limone oxide, overview. Enzyme Tomsk-LEH shows stereospecificity in the hydrolysis of cis/trans mixtures of (+)-limonene oxide by preferring the trans isomer | uncultured organism | (1S,2S,4R)-limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | enantiomeric mixtures of (+)-limone oxide, overview. Re-LEH catalyzes the quantitative conversion of the (+)- and (-)-limonene oxide cis/trans mixtures into the respective diols, the (+)-enantiomer is the better substrate | Rhodococcus erythropolis | (1S,2S,4R)-limonene-1,2-diol + (1R,2R,4S)-limonene-1,2-diol | - |
? | |
limonene-1,2-epoxide + H2O | enantiomeric mixtures of (+)-limone oxide, overview. Re-LEH catalyzes the quantitative conversion of the (+)- and (-)-limonene oxide cis/trans mixtures into the respective diols, the (+)-enantiomer is the better substrate | Rhodococcus erythropolis DCL14 | (1S,2S,4R)-limonene-1,2-diol + (1R,2R,4S)-limonene-1,2-diol | - |
? | |
additional information | commercially available cis/trans mixtures of (+)-limonene oxide (59:41 mixture of (1R,2S,4R) and (1S,2R,4R)) and (-)-limonene oxide (55:45 mixture of (1S,2R,4S) and (1R,2S,4S)) are dissolved in CH3CN and diluted with the appropriate LEH-containing buffer solution | Rhodococcus erythropolis | ? | - |
? | |
additional information | commercially available cis/trans mixtures of (+)-limonene oxide (59:41 mixture of (1R,2S,4R) and (1S,2R,4R)) and (-)-limonene oxide (55:45 mixture of (1S,2R,4S) and (1R,2S,4S)) are dissolved in CH3CN and diluted with the appropriate LEH-containing buffer solution | uncultured organism | ? | - |
? | |
additional information | commercially available cis/trans mixtures of (+)-limonene oxide (59:41 mixture of (1R,2S,4R) and (1S,2R,4R)) and (-)-limonene oxide (55:45 mixture of (1S,2R,4S) and (1R,2S,4S)) are dissolved in CH3CN and diluted with the appropriate LEH-containing buffer solution | Rhodococcus erythropolis DCL14 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CH55-LEH | - |
uncultured organism |
LEH | - |
Rhodococcus erythropolis |
LEH | - |
uncultured organism |
limA | - |
Rhodococcus erythropolis |
limA | - |
uncultured organism |
Re-LEH | - |
Rhodococcus erythropolis |
Tomsk-LEH | - |
uncultured organism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
uncultured organism |
50 | - |
- |
Rhodococcus erythropolis |
50 | - |
- |
uncultured organism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodococcus erythropolis |
8 | - |
assay at | uncultured organism |