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Literature summary for 3.3.2.6 extracted from

  • Seipelt, R.L.; Bailey, F.C.; Schaible, A.; Thompson, M.W.
    Asparagine362 is essential for zinc binding and catalysis in the peptidase reaction of Saccharomyces cerevisiae leukotriene A4 hydrolase (2010), Biochim. Biophys. Acta, 1804, 2070-2076.
    View publication on PubMed
Search for more literature for 3.3.2.6

Protein Variants

Protein Variants Comment Organism
D399A little effect on catalysis Saccharomyces cerevisiae
N362E large reduction in activity, mutant exhibits an altered pH dependence of catalysis, reduced chloride activation, and reduced zinc affinity and content Saccharomyces cerevisiae
N362L large reduction in activity, mutant exhibits an altered pH dependence of catalysis, reduced chloride activation, and reduced zinc affinity and content Saccharomyces cerevisiae
N362Q mutation causes minor catalytic defects Saccharomyces cerevisiae
W365L little effect on catalysis Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
 Saccharomyces cerevisiae
puromycin
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.105
-
 L-leucine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.128
-
 L-methionine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.137
-
 L-arginine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.15
-
 L-methionine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.166
-
 L-methionine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.199
-
 L-arginine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.235
-
 L-valine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.275
-
 L-arginine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.29
-
 L-leucine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.31
-
 L-alanine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.339
-
 L-alanine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.351
-
 L-methionine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.365
-
 L-leucine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.366
-
 L-arginine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.374
-
 L-methionine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.384
-
 L-valine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.4
-
 L-alanine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.488
-
 L-isoleucine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.49
-
 L-arginine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.566
-
 L-isoleucine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.587
-
 L-arginine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.618
-
 L-alanine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.657
-
 L-isoleucine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.693
-
 L-leucine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.698
-
 L-isoleucine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.7
-
 L-methionine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.745
-
 L-leucine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.771
-
 L-leucine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.803
-
 L-valine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.808
-
 L-valine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.885
-
 L-alanine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
1.78
-
 L-alanine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-alanine + 2-naphthylamine
-
 ?
L-arginine 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-arginine + 2-naphthylamine
-
 ?
L-isoleucine 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-isoleucine + 2-naphthylamine
-
 ?
L-leucine 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-leucine + 2-naphthylamine
-
 ?
L-methionine 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-methionine + 2-naphthylamine
-
 ?
L-valine 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-valine + 2-naphthylamine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 L-arginine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.0005
-
 L-alanine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.001
-
 L-leucine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.003
-
 L-alanine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.003
-
 L-methionine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.004
-
 L-valine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.005
-
 L-arginine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.006
-
 L-leucine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.007
-
 L-leucine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.009
-
 L-methionine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.009
-
 L-isoleucine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.01
-
 L-isoleucine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.012
-
 L-arginine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.013
-
 L-alanine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.017
-
 L-isoleucine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.02
-
 L-arginine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.02
-
 L-valine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.02
-
 L-arginine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.02
-
 L-valine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.027
-
 L-methionine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.027
-
 L-isoleucine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.03
-
 L-alanine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.034
-
 L-valine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.039
-
 L-arginine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.065
-
 L-alanine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.098
-
 L-methionine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.109
-
 L-alanine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.15
-
 L-leucine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.15
-
 L-methionine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.159
-
 L-methionine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.17
-
 L-leucine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.225
-
 L-leucine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7 broad Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0024
-
 bestatin mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.0032
-
 bestatin mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.0036
-
 bestatin mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.004
-
 bestatin mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.0046
-
 bestatin wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.008
-
 bestatin mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
1.33
-
 puromycin mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
2.82
-
 puromycin mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
4.16
-
 puromycin wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
4.39
-
 puromycin mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
5.1
-
 puromycin mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
5.6
-
 puromycin mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
 L-arginine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.002
-
 L-alanine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.005
-
 L-alanine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.005
-
 L-leucine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.009
-
 L-arginine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.01
-
 L-alanine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.012
-
 L-isoleucine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.016
-
 L-valine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.018
-
 L-leucine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.025
-
 L-methionine 2-naphthylamide mutant N362L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.026
-
 L-isoleucine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.03
-
 L-isoleucine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.03
-
 L-valine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.032
-
 L-alanine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.04
-
 L-arginine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.042
-
 L-valine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.05
-
 L-valine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.052
-
 L-methionine 2-naphthylamide mutant N362E, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.055
-
 L-isoleucine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.057
-
 L-leucine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.073
-
 L-alanine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.09
-
 L-arginine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.117
-
 L-arginine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.141
-
 L-arginine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.177
-
 L-methionine 2-naphthylamide mutant N362Q, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.2
-
 L-leucine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.21
-
 L-methionine 2-naphthylamide wild-type, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.238
-
 L-leucine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.263
-
 L-methionine 2-naphthylamide mutant D399A, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.292
-
 L-leucine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.321
-
 L-alanine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae
0.453
-
 L-methionine 2-naphthylamide mutant W365L, 25°C, pH not specified in the publication Saccharomyces cerevisiae