Cloned (Comment) | Organism |
---|---|
into the pYES2.1-Topo vector for expression of the recombinant proteins in Saccharomyces cerevisiae 1971 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
Y244F | leukotriene A4 hydrolase mutant | Saccharomyces cerevisiae |
Y256F | leukotriene A4 hydrolase mutant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | - |
Saccharomyces cerevisiae | |
puromycin | - |
Saccharomyces cerevisiae | |
RB-3014 | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | Saccharomyces cerevisiae | substrate for epoxide hydrolase activity | (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using metal affinity chromatography, the His tag is removed by digestion with His6-TEV protease | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | substrate for epoxide hydrolase activity | Saccharomyces cerevisiae | (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
leukotriene-A4 hydrolase | - |
Saccharomyces cerevisiae |
LTA4H | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
activity assay | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000087 | - |
RB-3014 | wild-type LTA4H | Saccharomyces cerevisiae | |
0.00074 | - |
RB-3014 | mutant Y456F | Saccharomyces cerevisiae | |
0.0027 | - |
bestatin | wild-type LTA4H | Saccharomyces cerevisiae | |
0.0037 | - |
RB-3014 | mutant Y244F | Saccharomyces cerevisiae | |
0.049 | - |
bestatin | mutant Y456F | Saccharomyces cerevisiae | |
0.0765 | - |
bestatin | mutant Y244F | Saccharomyces cerevisiae | |
2.37 | - |
puromycin | mutant Y244F | Saccharomyces cerevisiae | |
3.16 | - |
puromycin | wild-type LTA4H | Saccharomyces cerevisiae | |
3.24 | - |
puromycin | mutant Y456F | Saccharomyces cerevisiae |