Application | Comment | Organism |
---|---|---|
medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
E296D | decrease of inhibitor potency of bestatin in comparison to wild-type enzyme, but the inhibitor potencies of alpha-oxo-beta-amino carboxylic ester, a thioamine and amino hydroxamic acid is identical for mutant and wild-type enzyme | Mus musculus |
E296N | decrease of inhibitor potency of bestatin in comparison to wild-type enzyme, but the inhibitor potencies of alpha-oxo-beta-amino carboxylic ester, a thioamine and amino hydroxamic acid is identical for mutant and wild-type enzyme | Mus musculus |
E296Q | decrease of inhibitor potency of bestatin in comparison to wild-type enzyme, but the inhibitor potencies of alpha-oxo-beta-amino carboxylic ester, a thioamine and amino hydroxamic acid is identical for mutant and wild-type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxo-3-amino carboxylic esters | comparison of inhibition of mutant and wild-type enzymes | Mus musculus | |
bestatin | comparison of inhibition of mutant and wild-type enzymes, mechanism | Mus musculus | |
hydroxamic acid | comparison of inhibition of mutant and wild-type enzymes | Mus musculus | |
thioamine | comparison of inhibition of mutant and wild-type enzymes | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | 0.005 | leukotriene A4 | pH 7.9, 22°C, mutant E296D | Mus musculus | |
0.005 | - |
leukotriene A4 | pH 7.9, 22°C, wild-type enzyme | Mus musculus | |
0.005 | - |
leukotriene A4 | pH 7.9, 22°C, mutant E296N | Mus musculus | |
0.011 | - |
leukotriene A4 | pH 7.9, 22°C, mutant E296Q | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc protein | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.31 | - |
mutant E296N | Mus musculus |
0.49 | - |
mutant E296D | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | - |
Mus musculus | leukotriene B4 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
leukotriene A4 | pH 7.9, 22°C, wild-type enzyme | Mus musculus | |
1.4 | - |
leukotriene A4 | pH 7.9, 22°C, mutant E296Q | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
bestatin | pH 7.9, 22°C, wild-type enzyme | Mus musculus | |
0.021 | - |
bestatin | pH 7.9, 22°C, mutant E296Q | Mus musculus |