Activating Compound | Comment | Organism | Structure |
---|---|---|---|
leukotriene A4 | stimulates strong aminopeptidase activity via lipid binding site | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in Sf9 cells, using the baculovirus system | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | - |
Homo sapiens | |
bestatin | aminopeptidase activity, leukotriene A4 competes partially | Saccharomyces cerevisiae | |
hydroxamic acid | aminopeptidase activity | Saccharomyces cerevisiae | |
leukotriene A4 | leukotriene A4 binds covalently to Tyr378 and blocks epoxide hydrolase and aminopeptidase activities | Homo sapiens | |
leukotriene A4 | inhibits epoxid hydrolase activity and stimulates peptidase activity | Saccharomyces cerevisiae | |
thioamine | - |
Homo sapiens | |
thioamine | aminopeptidase activity, leukotriene A4 restores | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
L-leucine-4-nitroanilide | pH 7.5, 22°C | Homo sapiens | |
1.5 | - |
L-leucine-4-nitroanilide | pH 7.5, 22°C | Saccharomyces cerevisiae | |
2 | - |
L-leucine-4-nitroanilide | pH 7.5, 22°C, enzyme stimulated by leukotriene A4 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc protein | Homo sapiens | |
Zinc | zinc protein | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | Homo sapiens | - |
leukotriene B4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Homo sapiens | |
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine-4-nitroanilide + H2O | - |
Homo sapiens | L-leucine + 4-nitroaniline | - |
? | |
L-leucine-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | L-leucine + 4-nitroaniline | - |
? | |
leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
leukotriene A4 + H2O | - |
Saccharomyces cerevisiae | (5S,6S)-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid | i.e. DHETE | ? | |
additional information | - |
Homo sapiens | ? | - |
? | |
additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | - |
Caenorhabditis elegans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Homo sapiens |
? | x * 72000, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
L-leucine-4-nitroanilide | pH 7.5, 22°C | Homo sapiens | |
0.63 | - |
L-leucine-4-nitroanilide | pH 7.5, 22°C | Saccharomyces cerevisiae | |
5.2 | - |
L-leucine-4-nitroanilide | pH 7.5, 22°C, enzyme stimulated by leukotriene A4 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Saccharomyces cerevisiae |