Literature summary for 3.3.2.6 extracted from

Mueller, M.J.; Andberg, M.B.; Samuelsson, B.; Haeggstrom, J.Z.
Leukotriene A4 hydrolase, mutation of tyrosine 378 allows conversion of leukotriene A4 into an isomer of leukotriene B4 (1996), J. Biol. Chem., 271, 24345-24348.

Search for more literature for 3.3.2.6

Application

Application	Comment	Organism
medicine	the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
Y378F	products are leukotriene B4 and 5S,12R-dihydroxy-6,10-trans-8,14-cis-eicosatetraenic acid, 203% hydrolase activity, 81% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme	Homo sapiens
Y378Q	products are leukotriene B4 and 5S,12R-dihydroxy-6,10-trans-8,14-cis-eicosatetraenic acid, 45% hydrolase activity, 3% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.006	-	leukotriene A4	pH 8.0, 22°C, wild-type enzyme	Homo sapiens
0.023	-	leukotriene A4	pH 8.0, 22°C, mutant Y378F	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
leukotriene A4 + H2O	Homo sapiens	biosynthesis of leukotriene B4	leukotriene B4	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
leukotriene A4 + H2O	-	Homo sapiens	leukotriene B4	-	?
leukotriene A4 + H2O	biosynthesis of leukotriene B4	Homo sapiens	leukotriene B4	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.85	-	leukotriene A4	pH 8.0, 22°C, wild-type enzyme	Homo sapiens
2.09	-	leukotriene A4	pH 8.0, 22°C, mutant Y378F	Homo sapiens

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Release 2023.1 (January 2023)