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Literature summary for 3.3.2.6 extracted from

  • Minami, M.; Bito, H.; Ohishi, N.; Tsuge, H.; Miyano, M.; Mori, M.; Wada, H.; Mutoh, H.; Shimada, S.; Izumi, T.; Abe, K.; Shimizu, T.
    Leukotriene A4 hydrolase, a bifunctional enzyme. Distinction of leukotriene A4 hydrolase and aminopeptidase activities by site-directed mutagenesis at Glu-297 (1992), FEBS Lett., 309, 353-357.
    View publication on PubMed
Search for more literature for 3.3.2.6

Application

Application Comment Organism
medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E297A 30% hydrolase activity, 25% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
E297D 17% hydrolase activity, 38% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
E297K 13% hydrolase activity, 16% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
E297Q 104% hydrolase activity, 14% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
E319K 0% hydrolase activity, 12% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
Y384F 0% hydrolase activity, 13% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc zinc protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
leukotriene A4 + H2O Homo sapiens biosynthesis of leukotriene B4 leukotriene B4
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + H2O
-
 Homo sapiens leukotriene B4
-
 ?
leukotriene A4 + H2O biosynthesis of leukotriene B4 Homo sapiens leukotriene B4
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 ?
additional information either glutamic or glutamine moiety at amino acid 297 is required for full LTA4 hydrolase activity, free carboxylic acid of glutamic acid is essential for aminopeptidase Homo sapiens ?
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 ?